We narrowed to 15,050 results for: pET

1. pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

   Plasmid

   #117909

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R98A on eDHFR

   Promoter

   T7

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-AMPKsub-YPet-Kras

   Plasmid

   #84638

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-Kras
   

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22-Strep-TEV-Nsp10

   Plasmid

   #154958

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   Strep-TEV

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET21a-hAIM2-PYD-GST

   Plasmid

   #51531

   Purpose

   Express GST-tagged PYD of hAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   AIM2 (AIM2 Human)
   

   Tags

   GST and T7

   Expression

   Bacterial

   Mutation

   deletion of amino acids 102-343

   Promoter

   lac operator

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)

   Plasmid

   #127636

   Purpose

   Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   mTurquoiuse2-Tat, YPet-PCP
   

   Use

   Synthetic Biology

   Tags

   RNA binding peptide: PCP and RNA binding peptide:…

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET11a-TAX-L10-STEPtag

   Plasmid

   #159375

   Purpose

   Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   TAX-L10-STEPtag
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET11a-STEPtag-L10-TAX

   Plasmid

   #159374

   Purpose

   Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   STEPtag-L10-TAX
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-ACP (from S. aureus)

   Plasmid

   #63686

   Purpose

   Inducible expression of Acyl Carrier Protein (ACP) from S. aureus

   Depositor

   Michael Johnson

   Insert

   ACP
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized

   Promoter

   T7

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET11d-FHis-DND1-Y102A

   Plasmid

   #70082

   Purpose

   Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (DND1 Human)
   

   Tags

   6xHIS and FLAG

   Expression

   Bacterial

   Mutation

   Y102A. Codon optimized for bacterial expression

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

    Plasmid

    #128278

    Purpose

    Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

    Depositor

    Anna Sokac

    Article

    Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

    Insert

    twinstar (tsr) (tsr Fly)
    

    Tags

    6X Histidine tag

    Expression

    Bacterial

    Mutation

    Met1 changed to Pro1; Cys77 silent basepair mutat…

    Promoter

    T7

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDRF1-GW ymYPET-T2A-mTurquoise2

    Plasmid

    #118444

    Purpose

    Produces equimolar expression of ymYPET and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymYPET-T2A-mTurquoise2
    

    Expression

    Yeast

    Mutation

    YPET A206K, F208S, E232L, N235D

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-DEST42-ARL6-V5-His6

    Plasmid

    #67422

    Purpose

    Expression of hArl6 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL6 (ARL6 Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. HOXC13(human) GSTfusion-pET

    Plasmid

    #8680

    Depositor

    Corey Largman

    Article

    Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

    Insert

    HOXC13 (HOXC13 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    makes protein by Coomassie blue staining

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. M26I DJ-1 TEV site pET15b

    Plasmid

    #60685

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pETNKI-his-3C-LIC-Amp

    Plasmid

    #108704

    Purpose

    Expression of a His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-Pc4CL-PhCHS-CmCHI

    Plasmid

    #73397

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    PhCHS
    CmCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b-NmCas9-Flag H588A

    Plasmid

    #109032

    Purpose

    H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Tags

    Flag Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b+PCNA3 R112C/T180C

    Plasmid

    #66182

    Purpose

    Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C, T180C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b+PCNA2 E105C/L171C

    Plasmid

    #66178

    Purpose

    Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C, L171C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-22HT-MSI1(7-192)

    Plasmid

    #60245

    Purpose

    Express the RBD of mouse MSI1 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains aa7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. M26L DJ-1 TEV site pET15b

    Plasmid

    #60686

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26L DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26L and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pET-DEST42-ARL4C-V5-His6

    Plasmid

    #67423

    Purpose

    Expression of hArl4c with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL7 (ARL4C Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pET-DEST42-ARL4-V5-His6

    Plasmid

    #67419

    Purpose

    Expression of hArl4 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4 (ARL4A Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    bp 105 A to T; silent mutation

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pET15b/EYFP-GgVcl 1-258

    Plasmid

    #46263

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-258 (VCL Chicken)
    

    Tags

    EYFP and His

    Expression

    Bacterial

    Mutation

    aa 1-258 (VD1)

    Promoter

    T7

    Available Since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pet 3d double tentacle delete CP

    Plasmid

    #13301

    Depositor

    John Cooper

    Article

    Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

    Insert

    capping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids)
    

    Expression

    Bacterial

    Mutation

    Capping protein alpha 1 (with 28 amino acids dele…

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pET-21a(+) SurE D9A

    Plasmid

    #12686

    Depositor

    SeWon Suh

    Article

    Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

    Insert

    SurE
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Aspartate 9 to Alanine

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

27. HOXA9 (murine) no-tags pET

    Plasmid

    #8493

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HoxA9 (Hoxa9 Mouse)
    

    Expression

    Bacterial

    Mutation

    makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pET22b-EC12-129/138

    Plasmid

    #47926

    Purpose

    Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    Mutations C9A, K129C, D138C

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-28b(+)-DR RecR(34-220)

    Plasmid

    #12618

    Depositor

    SeWon Suh

    Article

    Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

    Insert

    Deinococcus radiodurans RecR(34-220)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-33

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pet 3d alpha tentacle delete CP

    Plasmid

    #13299

    Depositor

    John Cooper

    Article

    Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

    Insert

    capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits (CAPZB Chicken)
    

    Expression

    Bacterial

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only