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We narrowed to 24,925 results for: crispr

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Showing: 2181 - 2200 of 24925 results

  2. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Publication
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  3. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Plasmid
    #176534
    Purpose
    Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:
    Insert
    CRISPR-associated IscB (Delaware Bay metagenome)
    Tags
    14xHis-MBP-bdSUMO and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    176534-plasmid-map-sequence-id-345508

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  8. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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  11. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

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  13. pTE4495

    Plasmid
    #80338
    Purpose
    mammalian expression MbCpf1 nuclease and Mb crRNA
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Mb crRNA
    MbCpf1
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Promoter
    CMV and human U6
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80338-plasmid-map-sequence-id-151161

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  18. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Publication
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Synthetic, Human)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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Showing: 2181 - 2200 of 24925 results