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HA-MBP-TEVpCS-ADGRB1 CTF-FLAG

Plasmid

#217681

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRB1 (ADGRB1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-926

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE HA Chk2 KD, dominant negative chk2

Plasmid

#41902

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

Chk2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D347A

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR P4-P1R-EGFP

Plasmid

#48348

Purpose

Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EGFP

Use

Gateway entry vector

Mutation

Contains a Kozak sequence. Does not contain a sto…

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pHSP90(G97D)-mOFP

Plasmid

#228195

Purpose

expressed human Hsp90 (G97D mutation) with a mOFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

Insert

HSP90AA1(G97D) (HSP90AA1 Human)

Tags

mOFP

Expression

Mammalian

Mutation

G97D mutation

Promoter

CMV

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL4 CTF-FLAG

Plasmid

#217709

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRL4 (ADGRL4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-406

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL3 CTF-FLAG

Plasmid

#217708

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRL3 (ADGRL3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-841

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pHochTypeIII

Plasmid

#214039

Purpose

Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Cas10

Use

CRISPR

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

Plasmid

#217713

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRA3 (ADGRA3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-743

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

Plasmid

#217712

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRA2 (ADGRA2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-752

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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