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Plasmid#215677PurposeEmpty repair plasmid for ChrI split hygromycinR landing padDepositorInsert5'HA + MCS + loxN + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS79 (eft-3p::Cas9 + sgRNA)
Plasmid#154839PurposeCas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGGDepositorInsertsgRNA
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS59
Plasmid#215680PurposeCas9 + guide plasmid targeting ChrI split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV BAF170-Flag
Plasmid#19142DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
NbV5-HALOTAG
Plasmid#201482PurposeIndirect detection of V5-tagged protein using the HaloTag protein labeling technology.DepositorInsertNbV5-HALOTAG
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-VP40
Plasmid#74421PurposeMammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusionDepositorInsertVP40
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX-RCC1-HA
Plasmid#106938PurposeTransient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)DepositorInserthuman RCC1-HA (RCC1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWPI-TFDP1
Plasmid#114299PurposeConstitutive expression of the TFDP1 proteinDepositorInsertTFDP1 (TFDP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-mEos2
Plasmid#168515PurposeExpression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rateDepositorInsertmito-mEos2
UseTagsmEos2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CX-nestinGFPas
Plasmid#32401DepositorInsertnestin promoter/enhancer GFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp7
Plasmid#154757PurposeExpression of SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp8
Plasmid#154758PurposeExpression of SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Mito-mRFP
Plasmid#169001PurposeExpress Mito (Su9)-mRFP in zebrafishDepositorInsertMito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)
UseUnspecifiedTagsmRFPExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE478G-EGFP
Plasmid#68848Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationE478G, associated with ALSPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)
Plasmid#193852PurposeEncodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGGDepositorInsertGuide RNA
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FCK-Halo-GFP
Plasmid#14750PurposeLentiviral expression of Halo-GFP for neuronal inhibitionDepositorInsertMammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
UseLentiviralTagsGFPExpressionMammalianMutationN. pharaonis halorhodopsin is mammalian codon-opt…PromoterCaMKIIaAvailable sinceApril 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNP2157
Plasmid#222732PurposepNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 RecombinaseDepositorInsertIS621 Helper (WT)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG roGFP2-mito
Plasmid#168509PurposeExpression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potentialDepositorInsertmito-roGFP2
UseTagsroGFP2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS158
Plasmid#215679PurposeEmpty repair plasmid for ChrIII split hygromycinR landing padDepositorInsert5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP
Plasmid#226515PurposeMamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFPDepositorInsertCox8a(4x)-SNAP-tag2-T2A-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD474N-EGFP
Plasmid#68847Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationD474N (not linked to any diseases)PromoterCMVAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pR96L-EGFP
Plasmid#68846Purposemutated OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationR96L, associated with ALSPromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3XP3.RFP-tan
Plasmid#83474PurposeRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsert3XP3.RFP-tan
UseCRISPRTagsExpressionBacterial and InsectMutationPromoter3XP3 eye-expression PromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-mBmi1
Plasmid#114317PurposeTET-inducible expression of the mBmi-1 proteinDepositorInsertmBmi1 (Bmi1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
cr3Ec
Plasmid#184860Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination systemDepositorInsertCas9, gam, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rix-PGK-Tom-W
Plasmid#25813DepositorInsertRix-PGK-Tom-W
UseLentiviralTagsExpressionMutationtdTomato from Tsien labPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNP555
Plasmid#222722PurposesfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)DepositorTypeEmpty backboneUseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mTagBFP-T2A-TetOn3G
Plasmid#179888PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsmTagBFPExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pARR5::LUC
Plasmid#141170PurposeLuminescent reporter for cytokinin signalingDepositorInsertAtARR5 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtARR5, AT3G48100Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPTN217-577-EGFP
Plasmid#68841Purposedeleted OPTN was inserted into pEGFP-N1 backboneDepositorInsertoptineurin (OPTN Human)
UseTagsEGFPExpressionMammalianMutationaa 210-577 onlyPromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only