We narrowed to 22,070 results for: ESC

1. pRSETA-qmTQ2-citrate

   Plasmid

   #225251

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-citrate
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4046852

   Plasmid

   #145584

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pTubulin-miRFP670nano

   Plasmid

   #127429

   Purpose

   Human alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano-human alpha-tubulin
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLifeAct-miRFP713

   Plasmid

   #136562

   Purpose

   F-actin labeling with a near-infrared fluorescent protein miRFP713

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   LifeAct-miRFP713
   

   Use

   Tags

   miRFP713

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKB817 (pBra-Hfq)

   Plasmid

   #174661

   Purpose

   Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

   Depositor

   Ann Hochschild, Katherine Berry

   Article

   Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

   Insert

   Hfq
   

   Use

   Tags

   alpha NTD

   Expression

   Bacterial

   Mutation

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPaxillin-Electra2

   Plasmid

   #184926

   Purpose

   Expresses paxillin fused to blue fluorescent protein Electra2

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   paxillin-Electra2
   

   Use

   Tags

   Electra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. P305-FBP_4

   Plasmid

   #139695

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LAMP-CeNL_pcDNA3

   Plasmid

   #89514

   Purpose

   Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   CeNL
   

   Use

   Tags

   LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046979

   Plasmid

   #145611

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCMB-TMEr

    Plasmid

    #61208

    Purpose

    Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-mCherry-BCP
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. BRAC/pcDNA3

    Plasmid

    #51967

    Purpose

    mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

    Insert

    BRAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

12. LAMP-ReNL_pcDNA3

    Plasmid

    #89532

    Purpose

    Red color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046920

    Plasmid

    #145717

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCMV-NHERF1-PDZ1-2

    Plasmid

    #28294

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF1 (NHERF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains aa 1-298 (deletion of the EB domain aa 2…

    Promoter

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid

    #133317

    Purpose

    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus thermophilus #3)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    xylose

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQC-FsCFP-mCherryFP

    Plasmid

    #154836

    Purpose

    Frame-shift fluorescent reporter for genomic DNA editing

    Depositor

    Fangliang Zhang

    Article

    Kumar et al BMC Genomics. 2019 Jul 24;20(1):609. doi

    Insert

    targeting sequence of VEGF
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas12j2

    Plasmid

    #188498

    Purpose

    Expresses FLAG tagged Cas12j2

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LD-RA

    Plasmid

    #209863

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. DGK zeta

    Plasmid

    #35410

    Depositor

    Robert Lefkowitz, Stephen Prescott

    Article

    Nelson et al Science. 2007 Feb 2;315(5812):663-6.

    Insert

    DGK zeta (DGKZ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Mito-LSSmGFP

    Plasmid

    #186536

    Purpose

    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Use

    Tags

    Human COX8a first 29 amino acids

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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