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MSCVneo-HA-ER-Hoxb8

Plasmid

#222291

Purpose

The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

Mark Kamps, David Sykes

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pmiRFP709-N1

Plasmid

#79989

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE3G-Bi-α-chain

Plasmid

#133730

Purpose

Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Inserts

a-Chain_gePSI
ZsGreen1

Expression

Mammalian

Mutation

Only one part of the gePSI-system (aa1-141 + stop…

Promoter

pTRE3G-Bi

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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AGTR1-Tango

Plasmid

#66222

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

AGTR1 (AGTR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Sec8

Plasmid

#53758

Purpose

pEGFP-C3 plasmid that expresses Sec8

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec8 (EXOC4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 CDK7 D155A HA (P#812)

Plasmid

#14648

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Tags

HA

Expression

Mammalian

Mutation

D155A: dominant negative

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pDisplay FLIPE-600n

Plasmid

#13545

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

Plasmid

#90008

Purpose

Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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Ratiometric FPX sensor for ERK kinase activity

Plasmid

#60974

Purpose

For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A-WW domain-ERK substrate-ddFP B

Tags

ERK substrate is before ddFP copy B, WW domain is…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pVSVG-PATagRFP

Plasmid

#31947

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

VSVG

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag GRK5

Plasmid

#20495

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

GRK5 (GRK5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Lifeact-EGFP

Plasmid

#67303

Purpose

Fluorescent indicator for F-actin

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Lifeact-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-Ezrin-N-14

Plasmid

#54090

Purpose

Localization: Focal Adhesions/Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Ezrin (EZR Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLNCX mouse src

Plasmid

#13658

Depositor

Joan Brugge

Insert

src (Src Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pmGFP-P2A-K(AAG)20-P2A-RFP

Plasmid

#105689

Purpose

Double fluorescent stalling reporter K(AAG)20

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAG)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-CAD

Plasmid

#46238

Purpose

For expression of HA and FLAG-tagged murine CAD in mammalian cells

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEasy Flox

Plasmid

#11725

Depositor

Klaus Rajewsky

Article

Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

Insert

pEasyFlox

Use

Cre/Lox

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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mEmerald-mDia1-N-14

Plasmid

#54157

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

mDia1 (DIAPH1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-hRabin8 WT

Plasmid

#26726

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
flag-hKLF6 (1006)

Plasmid

#49488

Purpose

expresses human flag tagged KLF6 in mammalian cells

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

KFL6 (KLF6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only