We narrowed to 4,048 results for: bre

1. pcDNA3.1-Flag-stm2585-gp130

   Plasmid

   #174393

   Purpose

   Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA-IL6ST chimera
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   140-177 of sarA replaced with homologous IL6ST se…

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Flag-stm2585_Y167F

   Plasmid

   #174392

   Purpose

   S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   stm2585
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Tyr 167 changed to Phe

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK2 3'UTR KGA

   Plasmid

   #110402

   Purpose

   Luminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporter

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330.puro sgRNA KGA Stop1

   Plasmid

   #110405

   Purpose

   CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

   Plasmid

   #110408

   Purpose

   Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIGc-Xph18

   Plasmid

   #133029

   Purpose

   Expresses Xph18 in bacteria

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

   Insert

   Xph18
   

   Use

   Tags

   His10

   Expression

   Bacterial

   Mutation

   S63K

   Promoter

   T7

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AcrIIC1-mCherry chimera #10

   Plasmid

   #128113

   Purpose

   Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

   Depositor

   Dominik Niopek

   Article

   Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

   Insert

   AcrIIC1-mCherry chimera #10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIGc-Xph20

   Plasmid

   #133024

   Purpose

   Expresses Xph20 in bacteria

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

   Insert

   Xph20
   

   Use

   Tags

   His10

   Expression

   Bacterial

   Mutation

   S63K

   Promoter

   T7

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIGc-Xph15

   Plasmid

   #133030

   Purpose

   Expresses Xph15 in bacteria

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

   Insert

   Xph15
   

   Use

   Tags

   His10

   Expression

   Bacterial

   Mutation

   S63K

   Promoter

   T7

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. A3H HapII R175/6E-Cas9n-UGI

    Plasmid

    #119142

    Purpose

    Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A3H Hap II RR175/6EE

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEN_TT 3xFLAG SMAD4

    Plasmid

    #83094

    Purpose

    Gateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    SMAD4 (SMAD4 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    TRE tight promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

12. AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

    Plasmid

    #120300

    Purpose

    AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. IL1RN gRNA4

    Plasmid

    #113133

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-Rab11-WT

    Plasmid

    #101047

    Purpose

    Expresses HA-tagged human Rab11-WT in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    RAB11 (RAB11A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

    Plasmid

    #110854

    Purpose

    Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    sgRNA scaffold with spacer
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    U6

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPC-Flag-SOCS1

    Plasmid

    #129514

    Purpose

    Retroviral vector for the expression of human SOCS1 with a FLAG tag in N-terminal

    Depositor

    Gerardo Ferbeyre

    Article

    Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

    Insert

    SOCS1 (SOCS1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Rab27B

    Plasmid

    #89447

    Purpose

    Expression of GFP-tagged human Rab27B in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B (RAB27B Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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