We narrowed to 2,630 results for: organization Addgene

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TVBB C-term-mNeongreen-Puro

Plasmid

#169228

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mCh-KIF17*-strep

Plasmid

#120167

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 17 (KIF17 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF17: 1-509 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF13A*-strep

Plasmid

#120166

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 13A (KIF13A Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF13A: 1-639 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-SBP-VAP-A*

Plasmid

#120175

Purpose

Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

VAMP associated protein A (VAPA Human)

Tags

EGFP and SBP

Expression

Mammalian

Mutation

Truncated VAP-A: 118-249

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen-Blast

Plasmid

#169229

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen-P2A-Blast

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template

Plasmid

#167971

Purpose

Tp63 reporter targeting repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

Tp63 (TP63 Human)

Use

CRISPR; Donor template

Tags

Venus

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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PEX-mCherry-CRY2

Plasmid

#102250

Purpose

Expresses fusion of CRY2PHR with mCherry and PEX3 (1-42)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

PEX-mCherry-CRY2 (PEX3, CRY2 Mustard Weed, Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2-Hygro

Plasmid

#169225

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Hygro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2-Puro

Plasmid

#169224

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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SOX2 KO (T2A-H2B-EGFP)

Plasmid

#167969

Purpose

SOX2 CDS replacement with T2A-H2B-EGFP

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SRY-Box Transcription Factor 2 (SOX2 Human)

Use

CRISPR; Donor template

Tags

H2B-EGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowG-AURKA-mTurq2

Plasmid

#157768

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2-Puro

Plasmid

#169217

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMeCP2G pc1121

Plasmid

#167566

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Inserts

Methyl-CpG binding protein 2 (Mecp2 Rat)
GFP

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowY-AURKA-mTurq2

Plasmid

#157770

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowY

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 AURKA deltaNter

Plasmid

#157749

Purpose

Expression of AURKA without Nter in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

the first 30AA of Aurora A are lacking

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

Plasmid

#204189

Purpose

Doxycycline inducible lentiviral vector for human GATA6 overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSS02 NAPstar4.3

Plasmid

#241222

Purpose

Expresses NADPH/NADP+ biosensor NAPstar4.3 in Arabidopsis thaliana

Depositor

Markus Schwarzländer

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

cyt-NAPstar4.3

Expression

Plant

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSS02 NAPstar3

Plasmid

#241273

Purpose

Expresses NADPH/NADP+ biosensor NAPstar3 in Arabidopsis thaliana

Depositor

Markus Schwarzländer

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

cyt-NAPstar3

Expression

Plant

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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TRPML1-mCherry

Plasmid

#135189

Purpose

Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TRPML1 (Mcoln1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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