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pGAMA-empty

Plasmid

#74755

Purpose

Mammalian lentiviral vector

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-Magneto2.0-sNRPpA

Plasmid

#74307

Purpose

Cre-dependent expression of Magneto2.0 in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)

Use

AAV and Cre/Lox

Tags

Flag

Expression

Mammalian

Mutation

TRPV4: delta 760-871

Promoter

CMV (after Cre recombination)

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCR3.1-mIL2

Plasmid

#74467

Purpose

expression of mouse IL2 in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

IL2 (Il2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SLC38A9.1

Plasmid

#71855

Purpose

overexpression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGE011

Plasmid

#71537

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pQE30-hPolB

Plasmid

#70761

Purpose

bacterial expression of human DNA Polymerase beta (POLB)

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

POLB (POLB Human)

Tags

6x His

Expression

Bacterial

Mutation

R333L

Promoter

T5/lac operon

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSG7-Wt-NmeCas9

Plasmid

#71474

Purpose

bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-WT-NmeCas9

Tags

6His

Expression

Bacterial

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1_SEPT9 i1

Plasmid

#71622

Purpose

mammalian expression of human SEPT9 with an mCherry fusion

Depositor

Elias Spiliotis

Article

Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

Insert

Septin 9 (SEPTIN9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCAM-kan-R4R3

Plasmid

#71275

Purpose

Binary destination vector for plant transformation. Kanamycin resistance.

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Type

Empty backbone

Use

Gateway Cloning

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO_FOXA1_#1

Plasmid

#70095

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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