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pJJF152_Phsp_Cas9

Plasmid

#163862

Purpose

heat shock-induced expression of Cas9 in C. elegans

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

Cas9, C. elegans codon optimized with intron and SV40_NLS

Use

CRISPR

Expression

Worm

Promoter

Phsp-16.48

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF143_SECGFP_sg1

Plasmid

#163864

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2082

Plasmid

#155162

Purpose

Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaFΔ[248-269])-(6xHis-TEV-MlaB)

Expression

Bacterial

Mutation

deleted amino acids 248-269 in MlaF

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1514

Plasmid

#155147

Purpose

Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1965

Plasmid

#155152

Purpose

Expresses MlaF(1-246)-(6 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1966

Plasmid

#155153

Purpose

Expresses MlaF(1-246)-(10 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1967

Plasmid

#155154

Purpose

Expresses MlaF(1-246)-(14 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140170

Purpose

Expresses a regulatable secretory cargo for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140169

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo(Orange)

Plasmid

#140157

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Orange)

Plasmid

#140158

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Crimson)

Plasmid

#140156

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Plasmid

#140155

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#115428

Purpose

Expresses a non-glycosylated regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCI SMN1

Plasmid

#72286

Purpose

SMN1 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN1 (SMN1 Human)

Expression

Mammalian

Mutation

exons 6-7-8 including introns

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pet-15b-ultraID

Plasmid

#172879

Purpose

expresses ultraID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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LAMP1-mGFP

Plasmid

#34831

Depositor

Esteban Dell'Angelica

Article

Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

Insert

LAMP1 (LAMP1 Human)

Tags

GSTGSTGSTGA linker, Kozak, and mGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-2-EWSR1

Plasmid

#46384

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

Insert

EWSR1 (EWSR1 Human)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pFEndoNA

Plasmid

#174724

Purpose

Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

Depositor

Jukka Finne

Article

Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

Insert

GFP-endoNA fusion protein

Tags

Histag

Expression

Bacterial

Promoter

T5

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Pam-FL

Plasmid

#42570

Depositor

Vijaya Ramesh

Article

Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

Insert

human MYCBP2 (MYCBP2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Pam-FL clone lacks alternatively spliced exons 52…

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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