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pAAV_hSyn-fDIO-somQuasAr6a-EGFP-P2A-sombC1C2TG

Plasmid

#183537

Purpose

Voltage indicator

Depositor

Karl Deisseroth

Article

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Insert

somQuasAr6a-EGFP-P2A-sombC1C2TG

Use

AAV

Mutation

None

Promoter

hSyn

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA

Plasmid

#176540

Purpose

Mammalian expression of nuclear localized OgeuIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

OgeuIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-EYFP

Plasmid

#35507

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-vCre

Plasmid

#55638

Purpose

EF1a-driven expression of VCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Insert

vCre

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130991

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG_GST-TEV-WIPI3

Plasmid

#223800

Purpose

Expression of recombinant protein for purification

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI3 (WDR45B Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Coff/FonEYFP

Plasmid

#55652

Purpose

Cre-off/Flp-on EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre off/flp on eyfp

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-PTPRZ CA domain 13058 (VL+VH) synNotch

Plasmid

#247517

Purpose

Expresses a synNotch receptor binding to PTPRZ

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to PTPRZ

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-PTPRZ CA domain 13059 (VL+VH) synNotch

Plasmid

#247519

Purpose

Expresses a synNotch receptor binding to PTPRZ

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to PTPRZ

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-NRCAM 12949 (VL + VH) synNotch

Plasmid

#247525

Purpose

Expresses a synNotch receptor binding to NRCAM

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to NRCAM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

Plasmid

#35510

Purpose

AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

Plasmid

#35500

Purpose

AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

Plasmid

#35512

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV2

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

Plasmid

#130990

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-eYFP-WPRE

Plasmid

#130988

Purpose

AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(ER).iGlucoSnFR2.H348H.HaloTag

Plasmid

#244074

Purpose

ER expression of green glucose sensor (high affinity) with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Use

AAV

Tags

Calreticulin and HaloTag

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.H348H.HaloTag

Plasmid

#244070

Purpose

Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Use

AAV

Tags

HaloTag

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244103

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMRXIB-mRuby3-LC3

Plasmid

#221738

Purpose

Stable expression of mRuby3-LC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

LC3B (Map1lc3b Rat)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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