We narrowed to 14,590 results for: pET

1. p-miniTurbo

   Plasmid

   #209636

   Purpose

   Expression miniTurbo in mammalian cells

   Depositor

   Zhou Xiang

   Article

   Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

   Insert

   miniTurbo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCL1333

   Plasmid

   #217966

   Purpose

   Bacterial expression of RAN with an N-terminal 6His tag and a TEV cleavage site.

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   RAN (RAN Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. K560GFP

   Plasmid

   #129761

   Purpose

   Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   GFP and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 6xHis-MBP-huBsCas12a

   Plasmid

   #174671

   Purpose

   Expresses 6xHis-MBP tagged BsCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huBsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC28-LIC-sfGFP-His

   Plasmid

   #165479

   Purpose

   pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

   Depositor

   Renato Bruni

   Article

   Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

   Type

   Empty backbone

   Use

   Tags

   TEV-GFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 6xHis-MBP-huBoCas12a

   Plasmid

   #174673

   Purpose

   Expresses 6xHis-MBP tagged BoCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huBoCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 6xHis-MBP-huPb2Cas12a

   Plasmid

   #174686

   Purpose

   Expresses 6xHis-MBP tagged Pb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huPb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNIC-CTHF

   Plasmid

   #26105

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Jun 10. ():.

   Type

   Empty backbone

   Use

   Tags

   TEV - His6 - Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PIA900

   Plasmid

   #104401

   Purpose

   Polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA, B, C, and Z
   

   Use

   E. coli expression vector

   Tags

   encodes a C-terminally His-tagged rpoC which can …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCri-1b

    Plasmid

    #61327

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and MBP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pNIC-ZB

    Plasmid

    #26107

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Jun 10. ():.

    Type

    Empty backbone

    Use

    Tags

    His6 - Z-basic - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKEW397_AcrIIA15

    Plasmid

    #138285

    Purpose

    Expression of AcrIIA15 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA15
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKEW394_AcrIIA14

    Plasmid

    #138283

    Purpose

    Expression of AcrIIA14 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA14
    

    Use

    CRISPR

    Tags

    SFGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAM282

    Plasmid

    #226121

    Purpose

    Expression of cdc48

    Depositor

    Andreas Martin

    Article

    Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

    Insert

    His6-3C-Cdc48 (CDC48 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTSA-C

    Plasmid

    #17329

    Depositor

    Takeshi Sano

    Article

    Reznik et al Bioconjug Chem. . 12(6):1000-4.

    Insert

    streptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated to include only amino acids 16-133 of m…

    Promoter

    Available since

    Feb. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pLysscys255

    Plasmid

    #36429

    Depositor

    Emine Gunhan, Pete Lollar

    Article

    Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

    Insert

    Saporin-3/cys255
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cys added at position 255

    Promoter

    T7

    Available since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KRigor980

    Plasmid

    #129763

    Purpose

    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    R210A

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBD-0001

    Plasmid

    #12373

    Depositor

    Axel Brunger

    Article

    DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

    Insert

    VCP (Vcp Mouse)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. K560_AviC_coBirA

    Plasmid

    #129765

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUG-124

    Plasmid

    #104635

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
    

    Use

    Tags

    HIS-Ubq, HA-E2, GST-E3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDJSVT91

    Plasmid

    #109305

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    SufI 1-30, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBD-0016

    Plasmid

    #12345

    Depositor

    Axel Brunger

    Article

    DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

    Insert

    VCP R313K (Vcp Mouse)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    R313K, I206V*(see below)

    Promoter

    Available since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pCri-13a

    Plasmid

    #61321

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and MISTIC-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. K560_AviN_coBirA

    Plasmid

    #129766

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDJSVT89

    Plasmid

    #109303

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    TamA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKEW215_AcrIIA13

    Plasmid

    #138281

    Purpose

    Expression of AcrIIA13 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA13
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p2CT-Cdi

    Plasmid

    #83044

    Purpose

    Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

    Depositor

    Jennifer Doudna

    Article

    Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

    Insert

    CdiCas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

29. 6xHis-MBP-huHkCas12a

    Plasmid

    #174683

    Purpose

    Expresses 6xHis-MBP tagged HkCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huHkCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. KaiB-FLAG

    Plasmid

    #41885

    Depositor

    Michael Rust

    Article

    Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

    Insert

    Kai B
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    t7 promoter

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pCri-8a

    Plasmid

    #61317

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pCri-4b

    Plasmid

    #61328

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and TRX-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pCri-11b

    Plasmid

    #61333

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

34. K406

    Plasmid

    #129764

    Purpose

    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Truncation at 406

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Human Mevalonate Kinase (MK)

    Plasmid

    #52429

    Purpose

    bacterial expression of human Mevalonate Kinase (MK)

    Depositor

    Henry Miziorko

    Article

    Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

    Insert

    Human Mevalonate Kinase (MVK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    see comments

    Promoter

    T7

    Available since

    April 10, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pUG-301

    Plasmid

    #104730

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC10_EL3
    

    Use

    Tags

    HIS-Ubq, HA-E2

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 6xHis-MBP-huPcCas12a

    Plasmid

    #174681

    Purpose

    Expresses 6xHis-MBP tagged PcCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPcCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 6xHis-MBP-huCMtCas12a

    Plasmid

    #174680

    Purpose

    Expresses 6xHis-MBP tagged CMtCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huCMtCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCri-4a

    Plasmid

    #61314

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and TRX-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

40. 6xHis-MBP-huPdCas12a

    Plasmid

    #174682

    Purpose

    Expresses 6xHis-MBP tagged PdCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPdCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge