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We narrowed to 7,855 results for: 11

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Showing: 5551 - 5600 of 7855 results

  2. VEGF120TAG

    Plasmid
    #200946
    Purpose
    Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation
    Depositor
    Elizabeth Kirby
    Article
    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103
    Inserts
    VEGF120TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    Use
    Lentiviral
    Tags
    6x HIS
    Mutation
    amber stop codon 101 bp from ATG
    Promoter
    Ef1a and hPGK
    Available Since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    200946-plasmid-map-sequence-id-396320

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  3. VEGF164TAG

    Plasmid
    #200947
    Purpose
    Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation
    Depositor
    Elizabeth Kirby
    Article
    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103
    Inserts
    VEGF164TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    Use
    Lentiviral
    Tags
    6x HIS
    Mutation
    amber stop codon 101 bp from ATG
    Promoter
    Ef1a and hPGK
    Available Since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    200947-plasmid-map-sequence-id-396321

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  5. pINSPECT:NEAT1-total_SigP:NLuc

    Plasmid
    #197267
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-total homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197267-plasmid-map-sequence-id-400583

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  10. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid
    #114076
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R and K607R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114076-plasmid-map-sequence-id-220531

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  11. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid
    #114074
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114074-plasmid-map-sequence-id-220510

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  13. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

    Plasmid
    #50912
    Purpose
    Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C and I730C; N-term deletion of aa13-222; C-t…
    Promoter
    CMV
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    50912-plasmid-map-sequence-id-76385

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  24. pET15b-At 14-3-3 omicron

    Plasmid
    #44926
    Depositor
    Robert Ferl
    Insert
    14-3-3 omicron (GRF11 Mustard Weed)
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 14, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. pET29b(+)_gp1(A11V)

    Plasmid
    #225131
    Purpose
    Phage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)
    Depositor
    Matej Butala
    Insert
    gp1 (A11V)
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Alanine 11 to Valine
    Promoter
    T7 promoter
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225131-plasmid-map-sequence-id-448443

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  26. pGEM-T ZfHoxd11a

    Plasmid
    #27544
    Depositor
    Victoria Prince
    Insert
    Hoxd11a (hoxd11a Zebrafish)
    Expression
    Bacterial
    Available Since
    Feb. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  31. KLHL11

    Plasmid
    #39019
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL11 (KLHL11 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pHH0103 FNBP3 WW domain #1

    Plasmid
    #104233
    Purpose
    Bacterial expression of WW domain #1 from FNBP3
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    FNBP3 WW domain #1 (PRPF40A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104233-plasmid-map-sequence-id-204803

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  35. PRDM11

    Plasmid
    #32858
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM11 (PRDM11 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 79-314
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. pHH0103 FNBP3 WW domain #2

    Plasmid
    #104234
    Purpose
    Bacterial expression of WW domain #2 from FNBP3
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    FNBP3 WW domain #2 (PRPF40A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104234-plasmid-map-sequence-id-204379

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  42. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid
    #128589
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV8
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    Syn
    Available Since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128589-plasmid-map-sequence-id-257684

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  43. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190033
    Purpose
    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13X
    Expression
    Mammalian
    Mutation
    Y672A,Y676A
    Promoter
    CBh, SV40, hU6
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190033-plasmid-map-sequence-id-488812

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  44. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107942-plasmid-map-sequence-id-259760

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  46. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13d
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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  47. LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR (DRR728)

    Plasmid
    #248232
    Purpose
    Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attP target sites
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    None
    Promoter
    NA
    Available Since
    April 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    248232-plasmid-map-sequence-id-506813

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  48. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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  49. pAAV uN2CpolyGly op GFP (NIID)

    Plasmid
    #224356
    Purpose
    Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)
    Depositor
    Nicolas Charlet-Berguerand
    Article
    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.
    Insert
    uN2C with a GGC repeat expansion (100x)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Codon-optimized for expression in human, so no pu…
    Promoter
    CMV + chimeric intron (CAG)
    Available Since
    Feb. 5, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224356-plasmid-map-sequence-id-460492

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Showing: 5551 - 5600 of 7855 results