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pcDuex2-Skylan62A

Plasmid

#205755

Purpose

Expression of Skylan62A in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

Skylan62A

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBSMrnaSeph

Plasmid

#67507

Purpose

Express the tRNAMet scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pHIS3p:mRuby2-Tub1+3'UTR::URA3

Plasmid

#50639

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Promoter

HIS3p

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pEPIP_Peptide_Cloning_Vector

Plasmid

#172110

Purpose

Lentiviral plasmid for protein / peptide overexpression

Depositor

Prashant Mali

Article

Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

Type

Empty backbone

Use

Lentiviral

Promoter

Ef1-alpha

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mRuby2-Tub1+3'UTR::HIS3

Plasmid

#50657

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsFastLime

Plasmid

#205754

Purpose

Expression of rsFastLime in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsFastLime

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBSK

Plasmid

#67504

Purpose

Express the tRNALys scaffold

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys human

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDuex2-rScarlet

Plasmid

#205759

Purpose

Expression of rScarlet in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rScarlet

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsTagRFP

Plasmid

#205757

Purpose

Expression of rsTagRFP in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsTagRFP

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pFLAG UBR1 SBX

Plasmid

#24506

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 (UBR1 Budding Yeast)

Tags

FLAG

Expression

Yeast

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT_rHCaMP

Plasmid

#187107

Purpose

Expression of rHCaMP in mammalian cells

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

rHCaMP

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsFusionRed1

Plasmid

#205758

Purpose

Expression of rsFusionRed1 in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsFursionRed1

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mRuby2-Tub1+3'UTR::HPH

Plasmid

#50633

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Promoter

HIS3p

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pET51b_HCAII

Plasmid

#187110

Purpose

Expression of HCAII in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

HCAII

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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GST-PRMT2

Plasmid

#34650

Depositor

Steven Clarke

Article

Miranda et al Biochem Biophys Res Commun. 2005 Oct 28;

Insert

PRMT2 (PRMT2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBSKrnaSeph

Plasmid

#67506

Purpose

Express the tRNALys scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pRSET-B YFP

Plasmid

#108856

Purpose

Encodes for the fluorescent protein enhanced YFP (eYFP)

Depositor

Kalina Hristova

Article

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

eYFP

Expression

Bacterial

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLCRcala (p53)

Plasmid

#12136

Depositor

James Sherley

Article

Sherley et al J Biol Chem. 1991 Dec 25. 266(36):24815-

Insert

p53 (Trp53 Mouse)

Expression

Mammalian

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pMtBS

Plasmid

#32226

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

MCV Small t antigen

Expression

Mammalian

Mutation

Splice site was silently mutated, and a restricti…

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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