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pDest17_Gcn4 solvvol

Plasmid

#231877

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mCherry)

Plasmid

#233542

Purpose

A CMV promoter driving expression of a murine dominant negative myc tagged TCF4.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF4 (TCF4 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

lacks aa 1-56

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

Plasmid

#232128

Purpose

A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA2 (GATA2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#232124

Purpose

A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#232123

Purpose

A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#232129

Purpose

A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

Plasmid

#232131

Purpose

A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF2 (FOXF2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

Plasmid

#232130

Purpose

A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1036 (pME_V5_TCF7L2_P2A_H2A_mCherry)

Plasmid

#232126

Purpose

A middle entry gateway clone containing V5 tagged human TCF7L2 / TCF4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L2 / TCF4 (TCF7L2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-AP4-cyto

Plasmid

#229694

Purpose

Transient expression of EGFP AP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Control construct with tandem AP4 motifs (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-FP4-cyto

Plasmid

#229695

Purpose

Transient expression of FP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EYFP-Parkin

Plasmid

#210292

Purpose

mitophagy marker

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-Parkin

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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ECFP-FRB-GiantinS

Plasmid

#210296

Purpose

Golgi marker and anchor for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

ECFP-FRB-GiantinS

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP-FKBP-DN-N-WASP

Plasmid

#210301

Purpose

control for targeted actin nucleation

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-FKBP-DN-N-WASP

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP-FKBP-N-WASP

Plasmid

#210302

Purpose

targeted actin nucleation

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-FKBP-N-WASP

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)_L786A

Plasmid

#228838

Purpose

Insect cell expression of the mutant C-terminus of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800, with L786A

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)

Plasmid

#228833

Purpose

Insect cell expression of CC3 domain of BICD2 (aa 711-800)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(342-800)

Plasmid

#228832

Purpose

Insect cell expression of C-terminal portion of BICD2 (aa 342-800)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 342-800

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)_L742A

Plasmid

#228839

Purpose

Insect cell expression of the mutant C-terminus of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800, with L742A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-Rab5

Plasmid

#228139

Purpose

Expresses GFP-Rab5 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab5 (Rab5a Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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