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JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#232124

Purpose

A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#232123

Purpose

A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#232129

Purpose

A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

Plasmid

#232131

Purpose

A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF2 (FOXF2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

Plasmid

#232130

Purpose

A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1036 (pME_V5_TCF7L2_P2A_H2A_mCherry)

Plasmid

#232126

Purpose

A middle entry gateway clone containing V5 tagged human TCF7L2 / TCF4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L2 / TCF4 (TCF7L2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-AP4-cyto

Plasmid

#229694

Purpose

Transient expression of EGFP AP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Control construct with tandem AP4 motifs (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-FP4-cyto

Plasmid

#229695

Purpose

Transient expression of FP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EYFP-Parkin

Plasmid

#210292

Purpose

mitophagy marker

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-Parkin

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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ECFP-FRB-GiantinS

Plasmid

#210296

Purpose

Golgi marker and anchor for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

ECFP-FRB-GiantinS

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP-FKBP-DN-N-WASP

Plasmid

#210301

Purpose

control for targeted actin nucleation

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-FKBP-DN-N-WASP

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP-FKBP-N-WASP

Plasmid

#210302

Purpose

targeted actin nucleation

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-FKBP-N-WASP

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)_L786A

Plasmid

#228838

Purpose

Insect cell expression of the mutant C-terminus of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800, with L786A

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)

Plasmid

#228833

Purpose

Insect cell expression of CC3 domain of BICD2 (aa 711-800)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(342-800)

Plasmid

#228832

Purpose

Insect cell expression of C-terminal portion of BICD2 (aa 342-800)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 342-800

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)_L742A

Plasmid

#228839

Purpose

Insect cell expression of the mutant C-terminus of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800, with L742A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-Rab5

Plasmid

#228139

Purpose

Expresses GFP-Rab5 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab5 (Rab5a Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-SNAP-Omp25

Plasmid

#228137

Purpose

Expresses SNAP-Omp25 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Mutation

transmembrane region (C-terminus)

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28Man

Plasmid

#203399

Purpose

exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes

Depositor

David Vocadlo

Article

Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839

Insert

man2A

Tags

H6

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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TR2

Plasmid

#218403

Purpose

Expresses TR2 peptide in AAV9 capsid

Depositor

Casey Maguire

Article

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

TR2 7mer (RTTASLM)

Use

AAV

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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