We narrowed to 2,365 results for: DAP

1. pET24a-AlkB deltaN11

   Plasmid

   #73622

   Purpose

   Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

   Depositor

   Catherine Drennan

   Article

   Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   AlkB (alkB E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deletion of first 11 AA

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pX330-TJP1

   Plasmid

   #227299

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TJP1 (TJP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-M2-TLR2

   Plasmid

   #12291

   Depositor

   Bruce Beutler

   Article

   Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   TLR2 (Tlr2 Mouse)
   

   Use

   Tags

   FLAG (FLAG-M2)

   Expression

   Mammalian

   Mutation

   M82I and H202Y; signal peptide (aa1-23) replaced …

   Promoter

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pMJ825

   Plasmid

   #39315

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A)

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-AdEvolve-DEST

   Plasmid

   #131692

   Purpose

   A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-mStayGold-EFS-Zeo

   Plasmid

   #227258

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 CrkII

   Plasmid

   #50731

   Purpose

   mammalian expression of CrkII

   Depositor

   Kenneth Yamada

   Article

   Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

   Insert

   CrkII (CRK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-mCherry-LKB1(WT)

   Plasmid

   #84639

   Purpose

   mCherry-tagged, wild-type LKB1

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   mCherry-LBK1 (STK11 Synthetic, Human)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. EcNusGFL

   Plasmid

   #113122

   Purpose

   Expresses full-length E. coli NusG

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   NusG (nusG Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBMN-HA-NAP1

    Plasmid

    #208868

    Purpose

    For mammalian expression of NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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