We narrowed to 2,382 results for: T2A

1. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

   Plasmid

   #157974

   Purpose

   Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

   Depositor

   Carolyn McBride

   Article

   Zhao et al Nature. 2022 May;605(7911):706-712. doi:

   Insert

   Orco
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

   Plasmid

   #175261

   Purpose

   labelling of microtubule plus end and F-actin structures

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
   

   Use

   Tags

   mCherry (on iLifeact) and mNeonGreen (on EB3)

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pTub-alpha1

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR

   Plasmid

   #186740

   Purpose

   Dox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   RAB10, RABIF (RAB10, RABIF Human)
   

   Use

   Lentiviral

   Tags

   RAB10-T2A-RABIF-P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-mir144~451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164793

   Purpose

   Express miR-144~451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-144~451 (MIR451A, MIR144 Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170328

   Purpose

   Double-floxed inverse Orientation V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

   Plasmid

   #179843

   Purpose

   Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   eGFP and luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101102

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   TMt-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

   Plasmid

   #191675

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

   Depositor

   Veerle Baekelandt

   Inserts

   ATP10B (ATP10B Human)
   TMEM30A (TMEM30A Human)
   

   Use

   Lentiviral

   Tags

   6X his and flag

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry

   Plasmid

   #83490

   Purpose

   cre-dependent CA-AMPK under EF1a promoter

   Depositor

   Dong Kong

   Article

   Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

   Insert

   CA-AMPK-2A-mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   H150R

   Promoter

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo

    Plasmid

    #179439

    Purpose

    Expresses rTetR-KRAB(human) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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