Addgene

We narrowed to 11,638 results for: pcdna

Showing: 3361 - 3390 of 11638 results

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pcDNA3.1 Flag-TUBG2

Plasmid

#229855

Purpose

TUBG2

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

Insert

TUBG2 (TUBG2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Removal of the internal TUBG2-EcoRI site by chang…

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-SP1

Plasmid

#232649

Purpose

expresses SP1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP1 (SP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 N-T2A-M-IRES-E

Plasmid

#231903

Purpose

For the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' system

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, M, E SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

R203M in N protein

Promoter

CMV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FFSS-BACH1

Plasmid

#232273

Purpose

Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-BACH2

Plasmid

#232275

Purpose

Expression of FLAG-tagged BACH2 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH2 (BACH2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1a-FLAG

Plasmid

#232143

Purpose

Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1a (dgat1a Zebrafish)

Use

Zebrafish expression

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ mogat3b-FLAG

Plasmid

#232145

Purpose

Expresses zebrafish mogat3b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3b

Use

Zebrafish expression

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sga-Cas9

Plasmid

#227005

Purpose

Mammalian expression of Nuclease active S. gallolyticus Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sin-Cas9

Plasmid

#227006

Purpose

Mammalian expression of Nuclease active S. iniae Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Spa-Cas9

Plasmid

#227007

Purpose

Mammalian expression of Nuclease active S. parasanguinis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sub-Cas9

Plasmid

#227008

Purpose

Mammalian expression of Nuclease active S. uberis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-hMAPT

Plasmid

#231583

Purpose

Minigene of human MAPT containing exon 9, 10, 11 and relevant intronic sequences

Depositor

Chaolin Zhang

Article

Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

Insert

human MAPT (MAPT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nmEML-mRFP

Plasmid

#231178

Purpose

Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-15nmEML-mRFP

Plasmid

#231177

Purpose

Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

15nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, K98A)

Plasmid

#231377

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

H56A, K98A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A, K98A)

Plasmid

#231378

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R97A, K98A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97E, K98E)

Plasmid

#231380

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R97E, K98E

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A, H712A)

Plasmid

#231383

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R710A, H712A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-F-box:gs10:FRB:HA

Plasmid

#228531

Purpose

F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

F-box:gs10:FRB:HA (SKP2 Human, Synthetic)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S724A

Plasmid

#224735

Purpose

For expression of HA-tagged GTSE1 protein with S724A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 724 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S734A

Plasmid

#224736

Purpose

For expression of HA-tagged GTSE1 protein with S734A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 734 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S171A

Plasmid

#224723

Purpose

For expression of HA-tagged GTSE1 protein with S171A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 171 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S187A

Plasmid

#224724

Purpose

For expression of HA-tagged GTSE1 protein with S187A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 187 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S262A

Plasmid

#224725

Purpose

For expression of HA-tagged GTSE1 protein with S262A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 262 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S345A

Plasmid

#224726

Purpose

For expression of HA-tagged GTSE1 protein with S345A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 345 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S417A

Plasmid

#224727

Purpose

For expression of HA-tagged GTSE1 protein with S417A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 417 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S480A

Plasmid

#224728

Purpose

For expression of HA-tagged GTSE1 protein with S480A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 480 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S555A

Plasmid

#224729

Purpose

For expression of HA-tagged GTSE1 protein with S555A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 555 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-GTSE1-S594A

Plasmid

#224730

Purpose

For expression of HA-tagged GTSE1 protein with S594A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 594 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge