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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,812 results for: 129

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Showing: 5601 - 5650 of 5812 results

  12. pUDP044

    Plasmid
    #101168
    Purpose
    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101168-plasmid-map-sequence-id-199309

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  24. AAV-hSyn-scFVtet2_bGHpA

    Plasmid
    #177353
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    human Synapsine promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177353-plasmid-map-sequence-id-353612

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  25. AAV-TRE-scFVtet2_bGHpA

    Plasmid
    #177358
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    Tetracycline-dependent promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177358-plasmid-map-sequence-id-353663

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  32. pDM304_YFP-IqgC_mRFP-Raf1(RBD)

    Plasmid
    #128698
    Purpose
    simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells
    Depositor
    Igor Weber
    Article
    Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11
    Inserts
    iqgC (full length)
    Raf1 kinase (aa 50-132)
    Use
    Dictyostelium discoideum expression vector
    Tags
    YFP and mRFP
    Mutation
    none
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128698-plasmid-map-sequence-id-246776

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  35. pABD823

    Plasmid
    #67911
    Purpose
    Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    Cold temperature germinating 10 (CTG10)
    Tags
    GAL4 DNA binding domain (148 amino acids)
    Expression
    Yeast
    Promoter
    pADH1 (alcohol dehydrogenase 1 promoter)
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  36. pABD933

    Plasmid
    #67917
    Purpose
    Entry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    CTG10
    Use
    Gateway cloning vector
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  48. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid
    #212196
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40
    Depositor
    Stacey Edwards
    Article
    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:
    Insert
    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    Use
    CRISPR and Lentiviral
    Tags
    HA, NLS, and blasticidin S deaminase
    Expression
    Mammalian
    Promoter
    EF-1α promoter
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212196-plasmid-map-sequence-id-420116

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Showing: 5601 - 5650 of 5812 results