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pKMD106e - intein-Cas9(S219)

Plasmid

#64190

Purpose

Expresses intein-Cas9(S219) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-SN

Plasmid

#181741

Purpose

AAV vector for expressing SN

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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hSyn-GEM

Plasmid

#187648

Purpose

Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

PfV

Use

AAV

Tags

HaloTag and SNAP-tag

Expression

Mammalian

Promoter

Synapsin

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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EKARdual

Plasmid

#99870

Purpose

Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

EKAR

Tags

ShadowG and mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS-mCardinal2

Plasmid

#52631

Purpose

bacterial expression vector with far-red FP mCardinal2

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mCardinal2

Expression

Bacterial

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only

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gRNA-HEK3-Puro

Plasmid

#136282

Purpose

Expresses a guide RNA targeting HEK3 site

Depositor

Zhaohui Ye

Article

McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

Insert

sgRNA-HEK3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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p40phox-PX (1-144)

Plasmid

#119123

Purpose

Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

p40phox-PX (1-144)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B3-HA

Plasmid

#209228

Purpose

Mammalian expression of ATP8B3

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B3 (ATP8B3 Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET-His-VCre

Plasmid

#123131

Purpose

Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

VCre recombinase

Use

Cre/Lox

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAsCpf1(TYCV)(BB) (pY211)

Plasmid

#89352

Purpose

Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CBh

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_1

Plasmid

#103927

Purpose

Protein expression and purification of PDZK1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_4

Plasmid

#103945

Purpose

Protein expression and purification of PDZK1 PDZ4 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TJP2_PDZ_3

Plasmid

#103949

Purpose

Protein expression and purification of X104 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TJP2_PDZ_2

Plasmid

#103955

Purpose

Protein expression and purification of X104 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal TDP43 WT

Plasmid

#27458

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-null-GFPLVA

Plasmid

#23338

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
GAL FLAG25QDProGFP p306

Plasmid

#15831

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP3

Plasmid

#71467

Purpose

Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_2

Plasmid

#103943

Purpose

Protein expression and purification of PDZK1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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