We narrowed to 11,617 results for: SHA

1. 1.3xWT HBV-Luciferase

   Plasmid

   #61549

   Purpose

   The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core

   Depositor

   Yosef Shaul

   Article

   Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.

   Insert

   1.3 times of the HBV genome with luciferase within the Core ORF
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Substitution of HBV sequences with luciferase ORF…

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIP-AMPKa1_KD

   Plasmid

   #79011

   Purpose

   For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47R

   Promoter

   pCAGGs

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-SARS-Spike

   Plasmid

   #145031

   Purpose

   Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV Spike
   

   Use

   Tags

   C9 and CD5 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLAMP1-miRFP670nano

   Plasmid

   #127435

   Purpose

   LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   LAMP1-miRFP670nano
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPAmCherry-b-actin

   Plasmid

   #31949

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   b-actin

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   CMV

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pemiRFP703-N1

   Plasmid

   #136558

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP703
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

   Plasmid

   #202025

   Purpose

   Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpB S9G-R31L-R85L

   Promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 15x-QUAS-dTomato-T2A-GCaMP6s

   Plasmid

   #130666

   Purpose

   QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   15xQUAS-dTomato-T2A-GCaMP6s
   

   Use

   Ae. aegypti expression

   Tags

   Expression

   Insect

   Mutation

   Promoter

   15xQUAS-TATA

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTcI39-switch

   Plasmid

   #195557

   Purpose

   Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mRFP1
   mWasabi
   TcI39
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSYN-miRFP670nano3-WPRE-hGHpA

    Plasmid

    #184688

    Purpose

    Mammalian expression, miRFP670nano3

    Depositor

    Axel Nimmerjahn, Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSYN

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

    Plasmid

    #171437

    Purpose

    Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    c-Fos-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

    Plasmid

    #171438

    Purpose

    Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-c-Jun
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. EBFP2-Mito-7

    Plasmid

    #55248

    Purpose

    Localization: Mitochondria, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pmiRFP713-N1

    Plasmid

    #136559

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP713
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE-FLAG-LKB1

    Plasmid

    #8592

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH2B-miRFP703

    Plasmid

    #80001

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    histone cluster 1 H2B family member j (H2BC11 Human)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-hAgo2

    Plasmid

    #21981

    Depositor

    Phil Sharp

    Article

    Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-gRNA

    Plasmid

    #53062

    Purpose

    Transcription of guide RNA (gRNA) in wheat cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    wheat U6 promoter - gRNA
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    wheat U6

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRE-Slow-FT

    Plasmid

    #31915

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Slow-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

    Promoter

    PTight Tet-responsive PminCMVd

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF1a_mCherry_P2A_Hygro_Barcode

    Plasmid

    #120426

    Purpose

    Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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