Addgene

We narrowed to 3,080 results for: actin

Showing: 2241 - 2260 of 3080 results

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UAS-Myc-dASUN

Plasmid

#47036

Purpose

Drosophila expression of tagged dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Use

Tags

Myc

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
TV3-mCHY-dASUN

Plasmid

#47034

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Use

Tags

mCHY

Expression

Insect

Mutation

Promoter

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
TV3-eGFP-dASUN

Plasmid

#47033

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
pLG-Nde-Acc

Plasmid

#33150

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Use

Tags

LacZ

Expression

Yeast

Mutation

AccI site in intron was cut/filled/ligated to cha…

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE E171Q HDEL

Plasmid

#32124

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, E171Q

Promoter

Available since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE K108A GFP

Plasmid

#32126

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, K108A

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE K108A HDEL

Plasmid

#32122

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, K108A

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pMRX-IPU-FLAG-NEK9

Plasmid

#168269

Purpose

Expresses NEK9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

Plasmid

#170712

Purpose

dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Inserts

mNeonGreen
mScarlet

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TNNT2 and aMHC

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV4-NIK-HA

Plasmid

#27554

Depositor

Shao-Cong Sun

Article

Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

Insert

NIK (MAP3K14 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

NIK starts at amino acid 3; C52R and R139H mutati…

Promoter

CMV

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pJS183

Plasmid

#134142

Purpose

Bacterial expression of GFP-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-GFP-human Ska1; human Ska2

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-BmAgo3

Plasmid

#50559

Purpose

Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

BmAgo3

Use

Tags

6xHIS and Flag

Expression

Insect

Mutation

Promoter

OpIE2

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-IC2-FL

Plasmid

#51409

Purpose

expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

Depositor

Trina Schroer

Article

King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

Insert

dynein intermediate chain IC2C (Dync1i2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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TAK11

Plasmid

#227095

Purpose

Luciferase reporter vector with HIV-1 TAR region cloned in pGL3 Basic vector

Depositor

Farah Mustafa

Article

Khader et al Commun Biol. 2024 Nov 16;7(1):1521. doi:

Insert

CMV Promoter and HIV-1 TAR region

Use

Luciferase

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-Flag-UTR

Plasmid

#183235

Purpose

To express FLAG tagged eGFP

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

EGFP

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a-cys-His6-OtUBD

Plasmid

#190091

Purpose

Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRT498-3xOtUBD

Plasmid

#190088

Purpose

Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

3xOtUBD

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD

Plasmid

#190089

Purpose

Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGM1202

Plasmid

#69615

Purpose

inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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