We narrowed to 2,296 results for: pet28

1. pET-28a-His6-SUMO-RABIF

   Plasmid

   #135556

   Purpose

   Express human RABIF in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   RABIF (RABIF Human)
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK597

   Plasmid

   #71121

   Purpose

   Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

   Depositor

   T. J. Kappock

   Article

   Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

   Insert

   formyl-CoA:oxalate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tip alpha K102A/K104A (21-192)

   Plasmid

   #23159

   Depositor

   SeWon Suh

   Article

   Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

   Insert

   TNF-α-inducing protein
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed lysine 102 and lysine 104 to alanine, and…

   Promoter

   Available since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

4. p47 PX(1-121)/p40phox(136-339)

   Plasmid

   #19018

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox/p47phox (NCF4, NCF1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   insert containing amino acids 1-121 of p47phox fu…

   Promoter

   Available since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

5. PET-28a-YTH domain of YTHDC2

   Plasmid

   #228779

   Purpose

   Protein expression plasmid for YTH domain of YTHDC2 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDC2 (YTHDC2 Human)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SNAP-Kindlin

   Plasmid

   #186136

   Purpose

   For expression of recombinant Snap-labeled kindlin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   FERMT2 (FERMT2 Human)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

   Plasmid

   #89508

   Purpose

   Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

   Insert

   rAPOBEC1-nCas9 (D10A)-UGI-NLS
   

   Use

   Tags

   UGI and rAPOBEC-XTEN

   Expression

   Bacterial

   Mutation

   D10A mutation of Cas9

   Promoter

   T7

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB46 (CK2beta)

   Plasmid

   #27085

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpIMPDH

   Plasmid

   #206980

   Purpose

   Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

   Depositor

   Vern Schramm

   Article

   Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

   Insert

   SpIMPDH (guaB Streptococcus pyogenes)
   

   Use

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ALARM NMR

    Plasmid

    #90209

    Purpose

    Expresses the human La antigen for use in ALARM NMR

    Depositor

    Michael Walters

    Article

    Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi:

    Insert

    La antigen (SSB Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    contains amino acids 100-324

    Promoter

    T7 promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRD421

    Plasmid

    #163112

    Purpose

    Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
    

    Use

    Tags

    His-tag with TEV-cleavable linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXB1 (human) HIS-tag pET

    Plasmid

    #8520

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB1 (HOXB1 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    PCR from cDNA clone

    Promoter

    Available since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

13. HOXB3 (human) HIS-tag pET

    Plasmid

    #8524

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. N-His-GvpC-ssrA

    Plasmid

    #153297

    Purpose

    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    

    Use

    Tags

    His-tag and ssrA tag

    Expression

    Bacterial

    Mutation

    1) N-terminal 6xHis tag flanked by 1x glycine res…

    Promoter

    T7

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HOXD4 (murine) HIS-tag pET

    Plasmid

    #8548

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western and by in vitro transcri…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. HOXB9 (murine) HIS-tag pET

    Plasmid

    #8541

    Depositor

    Corey Largman

    Article

    Chang et al Mol Cell Biol 1996 Apr;16(4):1734-45.

    Insert

    HOXB9 (Hoxb9 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Missing the first 13 amino acids of HOXB9; makes …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. LARP1_323-509

    Plasmid

    #209800

    Purpose

    Bacterial expression of human LARP1 La-module

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 La-module (LARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-509 only

    Promoter

    T7

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LARP1_323-410

    Plasmid

    #209801

    Purpose

    Bacterial expression of human LARP1 LaM domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 LaM (LARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-410 only

    Promoter

    T7

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28a(+)-6xHis-TEV-ORF9c

    Plasmid

    #201023

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    open reading frame 9c
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)
    

    Use

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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