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178,119 results

Showing: 11381 - 11390 of 178119 results

  1. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68708-plasmid-map-sequence-id-123989

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  3. pcDNA3 AIF 1-90 mCherry FRB

    Plasmid
    #67530
    Purpose
    For expression of mCherry fused FRB to the mitochondrial innermembrane
    Depositor
    Stephen Tait
    Publication
    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    AIF a.a. 1 - 90
    mCherry
    FRB
    Tags
    AIF 1 - 90, FRB, and mCherry
    Expression
    Mammalian
    Mutation
    T->L
    Promoter
    CMV
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67530-plasmid-map-sequence-id-116204

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  4. Ii-Str_SBP-EGFP-Ecadherin

    Plasmid
    #65288
    Purpose
    synchronize trafficking of Ecadherin from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ecadherin
    Tags
    EGFP and IL-2 Signal Sequence and Streptavidin Bi…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65288-plasmid-map-sequence-id-120704

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  7. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65769-plasmid-map-sequence-id-112659

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  8. pBa-KIF5C 559-tdTomato-FKBP

    Plasmid
    #64211
    Purpose
    KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags
    Depositor
    Gary Banker, Marvin Bentley
    Publication
    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Kif5c (Kif5c Rat)
    Tags
    FKBP and tdTomato
    Expression
    Mammalian
    Mutation
    motor domain aa1-559
    Promoter
    chicken Beta Actin
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64211-plasmid-map-sequence-id-107628

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  10. Mucolipin1-pEGFP C3

    Plasmid
    #62960
    Purpose
    full length human Mucolipin-1 cloned into pEGFP C3
    Depositor
    Paul Luzio
    Publication
    Pryor et al Traffic. 2006 Oct;7(10):1388-98.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mucolipin-1 (MCOLN1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mucolipin-1 has 1 silent mutation compared to the…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 11381 - 11390 of 178119 results