We narrowed to 8,310 results for: Set;

1. pTE1062

   Plasmid

   #172356

   Purpose

   Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

   Depositor

   Eriko Takano

   Article

   Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

   Inserts

   scbR (SCO6265 )
   kasOp
   

   Use

   Synthetic Biology; Integrative into streptomyces …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPbuCas13b

   Plasmid

   #184565

   Purpose

   Inducible expression of PbuCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Prevotella buccae
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTF-lacZ

   Plasmid

   #153036

   Purpose

   For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKHR5

   Plasmid

   #74593

   Purpose

   an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.

   Depositor

   David Grunwald

   Article

   Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

   Insert

   FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   alpha-crystallin

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMARO

   Plasmid

   #178086

   Purpose

   Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

   Depositor

   Sören Abel

   Article

   Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

   Insert

   TcR (tetA/tetR)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Tetracycline resistance cassette is flanked by Ec…

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQdCas9-sgempty

   Plasmid

   #159523

   Purpose

   Expression of dCas9 driven by lux cassette from Vibrio fischeri

   Depositor

   Radhakrishnan Mahadevan

   Article

   Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eGFP-CBP

   Plasmid

   #169211

   Purpose

   Expression of eGFP-CBP protein for chitin visualization

   Depositor

   James S Fraser

   Article

   Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

   Insert

   Chitinase A1 Chitin Binding Domain
   

   Use

   Tags

   6xHis, Mxe Gyr Intein, and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. UMG-SP-1_pET29b

   Plasmid

   #215433

   Purpose

   Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. UMG-SP-2_pET29b

   Plasmid

   #215434

   Purpose

   Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS-KS-attB2-SA(1)-T2A-Gal4

    Plasmid

    #62900

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-KS-attB2-SA(2)-T2A-Gal4

    Plasmid

    #62901

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7C-HSPA1L

    Plasmid

    #177660

    Purpose

    Recombinant protein synthesis of human HSPA1L

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSPA1L (HSPA1L Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NylB_pET29b

    Plasmid

    #215414

    Purpose

    Expression construct for NylB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HPV-31neo

    Plasmid

    #153283

    Purpose

    Neomycin selectable HPV31 genome

    Depositor

    Elliot Androphy

    Article

    Gilson et al J Virol. 2020 Jul 30;94(16). pii: JVI.00

    Insert

    HPV-31 genome with Neo cassette
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    HindIII and CsiI site mutated from original Neo c…

    Promoter

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pY2

    Plasmid

    #218115

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pES134

    Plasmid

    #127551

    Purpose

    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

    Depositor

    Emmanuele Severi, Gavin Thomas

    Article

    Wu et al Microbiology (Reading). 2024 Mar;170(3).

    Insert

    F3-flanked spectinomycin resistance cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-T3-hCasD10A-pA

    Plasmid

    #51638

    Purpose

    Expresses Cas9-nickase in mammalian cells and zygotes.

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

    Insert

    Codon-optimized Cas9 D10A
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3

    Plasmid

    #67276

    Purpose

    RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

    Inserts

    GFP (mWasabi)
    hygro
    

    Use

    Tags

    F3 and Frt

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Tt-NylC_pET28a

    Plasmid

    #215431

    Purpose

    Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Tt-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFLAG-ING2

    Plasmid

    #13295

    Depositor

    Curtis Harris

    Article

    Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

    Insert

    ING2 (INGX Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only