We narrowed to 11,370 results for: Vars;

1. pCRISPR3_BC3

   Plasmid

   #191858

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR3_BC1

   Plasmid

   #191856

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ppst93-MCRmar-NG

   Plasmid

   #192766

   Purpose

   Expresses NG-tagged MCRmar in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Available Since

   Dec. 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Ppst93-MCRaeo-CA

   Plasmid

   #192765

   Purpose

   Expresses CA-tagged MCRaeo in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Available Since

   Dec. 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Ppst93-MCRaeo-NB

   Plasmid

   #192764

   Purpose

   Expresses NB-tagged MCRaeo in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Available Since

   Dec. 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Ppst93-MCRaeo-NG

   Plasmid

   #192763

   Purpose

   Expresses NG-tagged MCRaeo in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Available Since

   Dec. 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCK411.RR1

   Plasmid

   #192644

   Purpose

   Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   BBa_J23119-RR1
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   BBa_J23119

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR

   Plasmid

   #189943

   Purpose

   Lentiviral vector to co-express an spsgRNA with NLS-tagBFP

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TEG +H122D

   Plasmid

   #187434

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1
   ATP1B1
   

   Expression

   Insect

   Mutation

   changed histidine at 128 to aspartic acid

   Promoter

   PH and p10

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FER +H111E

    Plasmid

    #187429

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed histidine at 117 to glutamic acid

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. KEE +E111H

    Plasmid

    #187431

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed glutamic acid at 117 to histidine

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SNG +R111Q

    Plasmid

    #187437

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed arginine at 117 to glutamine

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FER +H122D

    Plasmid

    #187428

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed histidine at 128 to aspartic acid

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OST +Q111R

    Plasmid

    #187436

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed glutamine at 116 to arginine

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FER +H111T

    Plasmid

    #187430

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed histidine at 117 to threonine

    Promoter

    PH and p10

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CHI +N122D

    Plasmid

    #187427

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Expression

    Insect

    Mutation

    changed asparagine at 130 to aspartic acid

    Promoter

    PH and p10

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NODAL-MYC-DYK-N72A-N199A

    Plasmid

    #115252

    Purpose

    For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Tags

    MYC-DYK

    Expression

    Mammalian

    Mutation

    N72A, N199A

    Promoter

    CMV

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NODAL-MYC-DYK-C312A

    Plasmid

    #115253

    Purpose

    For mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Tags

    MYC-DYK

    Expression

    Mammalian

    Mutation

    C312A

    Promoter

    CMV

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDisplay-V5-Nxph1

    Plasmid

    #184336

    Purpose

    Expression of V5-tagged mouse neurexophilin-1

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    neurexophilin-1 (Nxph1 Mouse)
    

    Tags

    V5-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. GST_LIM1224

    Plasmid

    #182041

    Purpose

    For expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM1224 (PXN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    LIM domains 1, 2, 2 and 4

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. GST_LIM1334

    Plasmid

    #182040

    Purpose

    For expression of human paxillin LIM domains 1, 3, 3 and 4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM1334 (PXN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    LIM domains 1, 3, 3 and 4

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pML104-natR412

    Plasmid

    #162042

    Purpose

    Expresses Cas9 and contains natR412 guide which targets the natR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    natR412 guide
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pML107-natR854

    Plasmid

    #162043

    Purpose

    Expresses Cas9 and contains natR854 guide which targets the natR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    natR854 guide
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lmajor_gp63_10_0460_P460G_D463N_S465A

    Plasmid

    #171645

    Purpose

    Expression of L. major glcyoprotein-63 (P460G_D463N_S465A) with substrate binding site mutations from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

    Insert

    LmjF.10.0460 P460G D463N S465A
    

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    P460G; D463N; S465A

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJH191

    Plasmid

    #172149

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    

    Expression

    Bacterial

    Mutation

    gIII(P29*, Y184*); [strong promoter eG, strong RB…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pJH190

    Plasmid

    #172148

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    

    Expression

    Bacterial

    Mutation

    gIII(P29*); [strong promoter eG, strong RBS SD8] …

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. Negative feedback (NF) plasmid

    Plasmid

    #169747

    Purpose

    Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-tetO2

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMS11: pTns(AvCAST)_ΔTniQ,ΔTnsD

    Plasmid

    #168144

    Purpose

    Inducible expression of AvCAST TnsA, TnsB and TnsC proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMS9: pTns(AvCAST)_ΔTniQ

    Plasmid

    #168142

    Purpose

    Inducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLentiCRISPR V2 sgACO1_3

    Plasmid

    #169893

    Purpose

    Disrupt ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting ACO1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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