We narrowed to 2,376 results for: pet28

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pT7-HISx6-SUMOx10

Plasmid

#116937

Purpose

Bacterial expression vector for 10 sumo

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10 SUMO

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-SUMO-RNF168ring (1-189)

Plasmid

#63570

Purpose

Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

RNF168 (RNF168 Human)

Tags

6xHis and SUMO3

Expression

Bacterial

Mutation

Ring domain only (residues 1-189)

Promoter

T7

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
CFP-FRBx5-His

Plasmid

#103784

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

CFP-FRBx5-His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSunS

Plasmid

#171672

Purpose

SunS expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHsSR beta deltaN Flag

Plasmid

#13731

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRPRB Human)

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

expresses residues 59-271 of Homo sapiens SRβ wit…

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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YFP-FKBPx5-His

Plasmid

#103785

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-FKBPx5-His

Tags

EYFP, His-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-VAMP3

Plasmid

#135552

Purpose

Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP3

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a+GFP-CAMSAP2 CKK

Plasmid

#59032

Purpose

expresses CKK domain of CAMSAP2 in E. coli

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 CKK domain (CAMSAP2 Human)

Tags

His, Strep, TEV, and sfGFP

Expression

Bacterial

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pNIC28-Bsa4-ICL1

Plasmid

#105822

Purpose

Expression of Mycobacterium tuberculosis icl1 recombinant protein

Depositor

Ivanhoe Leung

Article

Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

Insert

icl1

Tags

His tag with TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a+GFP-CAMSAP1 CKK

Plasmid

#59031

Purpose

expresses CKK domain of CAMSAP1 in E. coli

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP1 CKK domain (CAMSAP1 Human)

Tags

His, Strep, TEV, and sfGFP

Expression

Bacterial

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET-28a-His6-SUMO-VAMP2

Plasmid

#135551

Purpose

Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP2

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET-GST-TNRC6A-(935-984)

Plasmid

#42050

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

GST and His

Expression

Bacterial

Mutation

contain aa935-984

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pMtb CblA

Plasmid

#138298

Purpose

Expression of Mtb CblA

Depositor

Ruma Banerjee

Article

Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

Insert

Mtb CblA

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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HpMscS

Plasmid

#78310

Purpose

H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

Depositor

Douglas Rees

Article

Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

Insert

Mechanosensitive channel of small conductance

Tags

6x His Tag and FLAG Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-6

Plasmid

#163132

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-VAMP8

Plasmid

#135553

Purpose

Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP8

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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p28atDarpp

Plasmid

#108318

Purpose

Expresses tDarpp in E.coli

Depositor

Jamil Momand

Article

Momand et al FEBS Open Bio. 2017 Aug 29;7(9):1328-133

Insert

tDarpp (PPP1R1B Human)

Tags

6 Histadine

Expression

Bacterial

Promoter

T7

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pZ004 (LseC2c2 locus)

Plasmid

#70170

Purpose

Expresses LseC2c2 locus with one spacer in array

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

LseC2c2 locus with one spacer in array

Expression

Bacterial

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
HOXB13 (human) HIS-tag pET

Plasmid

#8543

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Western and by transcription/tra…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET-NEMOL329P

Plasmid

#11969

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Tags

His

Expression

Bacterial

Mutation

Leucine329 to Proline

Available Since

July 13, 2006

Availability

Academic Institutions and Nonprofits only