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pET42a-lambdaN+-L+-GSH

Plasmid

#98894

Purpose

Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

lambdaN+-L+

Tags

GST tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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Atg24 1B

Plasmid

#197046

Purpose

His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Insert

Atg24

Tags

6His-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Smad7-HA

Plasmid

#13506

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Tags

HA

Expression

Bacterial

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
mpeg:Rab7-stop-polyA; cmlc2:GFP

Plasmid

#213738

Purpose

Expresses mCherry-Rab7 in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

mCherry-Rab7

Use

Zebrafish

Promoter

mpeg1.1

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5 MYC-Smurf2 (FF29/30A) WT

Plasmid

#24604

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A

Available Since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pJK_proB_eGFP

Plasmid

#59845

Purpose

Constitutively expresses eGFP with proB promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proB

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Cterm_His

Plasmid

#220483

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-URA3-3'SEC13-GFP

Plasmid

#25445

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

Insert

Sec13-Green Fluorescent Protein

Expression

Yeast

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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U6-Nme2_tracr

Plasmid

#192146

Purpose

Expresses Nme2-sgRNA cloned with BspQI

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

Nme2 sgRNA scaffold

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJK_proK1_eGFP

Plasmid

#59853

Purpose

Constitutively expresses eGFP with proK1 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK1

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Cterm_His

Plasmid

#220484

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-VPS35

Plasmid

#166220

Purpose

Gateway entry vector for N. furzeri VPS35

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

VPS35

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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CAG.3xHalo-GFP-SPC25

Plasmid

#176206

Purpose

Kinetochore anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

3xHalo-GFP-SPC25

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mEGFPBb

Plasmid

#118234

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mEGFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-HpENR

Plasmid

#12651

Depositor

SeWon Suh

Article

Lee et al Acta Crystallogr D Biol Crystallogr. 200

Insert

noyl-acyl carrier protein reductase (fabI Helicobacter pylori)

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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tGFP

Plasmid

#26864

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

Truncated GFP-IRES-Puro

Expression

Bacterial

Mutation

Deletion of nt 1-36

Available Since

Nov. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pXcX-3.6TPH-GAL4p65

Plasmid

#32573

Depositor

Sergey Kasparov

Article

Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

Insert

Gal4p65

Use

Adenoviral

Expression

Mammalian

Promoter

3.6TPH

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pIRES_iSeroSnFR_cytosolic

Plasmid

#214132

Purpose

Fluorescent biosensor for intracellular serotonin (5-HT)

Depositor

Steffen Sinning

Article

Bukowski et al Research Square PREPRINT. 18 August 2023

Insert

iSeroSnFR_cytosolic

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-600n

Plasmid

#13541

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0285

Plasmid

#11304

Depositor

Sung-Hou Kim

Article

Kim et al J Struct Biol. 1998 Jan . 121(1):76-80.

Insert

small heat shock protein (MJ_0285 M. jannaschii)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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