We narrowed to 12,677 results for: sequence

1. pT7-EGFP-C1-HsR3HCC1_39-480-EBMmut_K

   Plasmid

   #146765

   Purpose

   Mammalian Expression of HsR3HCC1_39-480-EBMmut

   Depositor

   Elisa Izaurralde

   Article

   Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

   Insert

   HsR3HCC1_39-480-EBMmut (R3HCC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence extracted from BC050572.1; 39-480 trunca…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-EGFP-C1-HsGIGYF1_1-177_AE

   Plasmid

   #148570

   Purpose

   Mammalian Expression of HsGIGYF1_1-177

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   HsGIGYF1_1-177 (GIGYF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-EGFP-C1-HsGIGYF1-Y39AY41AM46AL47A_AE

   Plasmid

   #148576

   Purpose

   Mammalian Expression of HsGIGYF1-Y39AY41AM46AL47A

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   HsGIGYF1-Y39AY41AM46AL47A (GIGYF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-HsGIGYF1_1-177-LFVENtoDDDAF_AE

   Plasmid

   #148581

   Purpose

   Mammalian Expression of HsGIGYF1_1-177-LFVENtoDDDAF

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   HsGIGYF1_1-177-LFVENtoDDDAF (GIGYF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENT-L1-Kozak-Ark-L2

   Plasmid

   #186362

   Purpose

   Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Ciona robusta Aurora kinase A
   

   Use

   Expression of a fluorescent centrosome marker

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. KTK_139

   Plasmid

   #180565

   Purpose

   Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. KTK_113

   Plasmid

   #180540

   Purpose

   Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   Signal peptide region of CsgA including SecB-N22 sequenced
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJCC_162 SpCas9 KES(1107-1109)GG

   Plasmid

   #179526

   Purpose

   For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 KES(1107-1109)GG
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   replaced KES (residues 1107-1109) with GG

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

   Plasmid

   #179527

   Purpose

   For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 K233A/K234A/K253A/K263A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed lysines 233, 234, 253, and 263 to alanine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. KTK_324

    Plasmid

    #180566

    Purpose

    Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPLN-hFluc-DM-HA-GFP11-KDEL

    Plasmid

    #177723

    Purpose

    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFlucDM
    

    Use

    Tags

    GFP11, HA, KDEL, and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    R188Q, R261Q (Destabilized Mutant)

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p406 – TDH3-ER-FlucDM-GFP11-Ura

    Plasmid

    #177731

    Purpose

    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    FlucDM
    

    Use

    Tags

    GFP11, HA, HDEL, and KAR2 Signal Sequence

    Expression

    Bacterial and Yeast

    Mutation

    R188Q, R261Q (Destabilized Mutant)

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p406 – TDH3-ER-FlucDM-GFP- Ura

    Plasmid

    #177732

    Purpose

    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    FlucDM
    

    Use

    Tags

    HA, HDEL, KAR2 Signal Sequence, and eGFP

    Expression

    Bacterial and Yeast

    Mutation

    R188Q, R261Q (Destabilized Mutant)

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Bmpr2 gRNA resistant

    Plasmid

    #163408

    Purpose

    Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    gRNA-targeting sequences are substituted with nuc…

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-10xMS2 no wild type

    Plasmid

    #158204

    Purpose

    Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites of MS2 without the WT sequence from oligo library experiment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-10xQb no wild type

    Plasmid

    #158205

    Purpose

    Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126758

    Purpose

    Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    evoSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126759

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126753

    Purpose

    Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126754

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge