We narrowed to 14,014 results for: TIM

1. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

   Plasmid

   #174091

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Promoter

   pCMV and T7

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yoTagRFP657-SpHis5

   Plasmid

   #44846

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoTagRFP657
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFH66

   Plasmid

   #131765

   Purpose

   Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJKR-O-acuR

   Plasmid

   #62569

   Purpose

   Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   CFP
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUB_FLuc100---MS2

   Plasmid

   #210574

   Purpose

   Expresses Optimal Firefly with 3'UTR MS2 hairpins

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Optimal Firefly luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   UBC human

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Twitch-1 pRSETB

   Plasmid

   #48207

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Mues et al Nat Med. 2013 Jun;19(6):778-83. doi: 10.

   Insert

   Twitch-1
   

   Tags

   6xHIS, ECFP, and cpCit174

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pMiniT:SEP-beta2 TKIT donor

   Plasmid

   #169436

   Purpose

   Donor DNA for TKIT knockin of SEP to Gabrb2 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cox8x2-ReNL_pcDNA3

   Plasmid

   #89533

   Purpose

   Red color variant of bright luminescent protein, targeted to the Mitochondria

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Tags

   CoxVIII X2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-link-yomApple-Kan

   Plasmid

   #44957

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mApple with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomApple
   

   Expression

   Yeast

   Available Since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. myr-palm_2xmEYFP

    Plasmid

    #127811

    Purpose

    Homo-dimer mEYFP-mEYFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mEYFP-mEYFP
    

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-link-yoLSS-mKate2-Kan

    Plasmid

    #44943

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoLSS-mKate2
    

    Expression

    Yeast

    Available Since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-link-yomKeima-Kan

    Plasmid

    #44902

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKeima
    

    Expression

    Yeast

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET FLIPglu-600uDelta13

    Plasmid

    #13559

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A Delta13
    

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    F16A in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAJM.717

    Plasmid

    #108517

    Purpose

    PTet*-YFP Reporter. aTc sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PTet*-YFP
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LBJJ382

    Plasmid

    #117518

    Purpose

    Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6-26

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAJM.712

    Plasmid

    #108513

    Purpose

    PCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PCymRC-YFP
    

    Expression

    Bacterial

    Available Since

    June 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTagRFP-pBAD

    Plasmid

    #54539

    Purpose

    Localization: Protein Expression Vector , Excitation: 555, Emission: 584

    Depositor

    Michael Davidson, Dmitriy Chudakov, Dorus Gadella

    Article

    Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

    Type

    Empty backbone

    Tags

    6xHis and mTagRFP

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pAJM.716

    Plasmid

    #108518

    Purpose

    PBAD-YFP Reporter. Ara sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PBAD-YFP
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEB1-mClover3

    Plasmid

    #103994

    Purpose

    Plasmid encoding mClover3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mClover3
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEB1-moxVenus

    Plasmid

    #103993

    Purpose

    Plasmid encoding moxVenus

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxVenus
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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