We narrowed to 54,439 results for: plasmid

1. RHOAA-c034

   Plasmid

   #205117

   Purpose

   RHOA protein expression

   Depositor

   Paul Brennan

   Insert

   RHOA (RHOA Human)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP12082 - pAAV-AiE0371m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2082)

   Plasmid

   #208127

   Purpose

   Direct-expressing SYFP2 in astrocytes AAV Virus

   Depositor

   Allen Institute for Brain Science, Boaz Levi

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)

   Plasmid

   #205417

   Purpose

   Mutagenesis of Ntsr1, second guide

   Depositor

   Larry Zweifel

   Insert

   Ntsr1 (Ntsr1 Mouse)
   

   Use

   AAV and CRISPR

   Promoter

   U6

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hPhyB621(Y276H)-P2A-m1V

   Plasmid

   #197377

   Purpose

   The AAV vector for PhyB621(Y276H) and m1V

   Depositor

   Michiyuki Matsuda

   Insert

   Phytochrome B Y276H(1-621 aa)
   

   Use

   AAV

   Tags

   P2A-m1V

   Mutation

   Human codon optimized PhyB (1-621 aa), Y276H muta…

   Promoter

   CMV

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Slack Slo2.2 max1-ECFP

   Plasmid

   #197318

   Purpose

   Plasmid expressing an antigen used to generate an antibody that targets the Slack K+ channel subunit

   Depositor

   Christopher Lingle

   Insert

   KCNT1/Slo2.2/Slack K+ channel (Kcnt1 Mouse)
   

   Tags

   ECFP

   Expression

   Mammalian

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

   Plasmid

   #182894

   Purpose

   Mammalian expression of PVALB rat mAb HC gene

   Depositor

   Heng Zhu

   Insert

   anti-PVALB (human) heavy chain (PVALB Rat)
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Mutation

   No

   Promoter

   CMV

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE3GV-somBiPOLES-mKate2

   Plasmid

   #192579

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   TRE3GV

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE3GV-somBiPOLES-mCerulean

   Plasmid

   #192578

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   TRE3GV

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CaMKII-somBiPOLES-eGFP

   Plasmid

   #192583

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of excitatory neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   CaMKIIa(0.4)

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ipUSEPR-sg-Hs-ACE2-A2

    Plasmid

    #188687

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgACE2 (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ipUSEPR-sg-Hs-ACE2-A4

    Plasmid

    #188688

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgACE2 (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ipUSEPR-sg-Hs-TMPRSS2-A5

    Plasmid

    #188689

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgTMPRSS2 (TMPRSS2 Human)
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. STX4AA-c005

    Plasmid

    #175487

    Purpose

    Protein expression in bacterial cells. Coiled-coil domains, M1-L275.

    Depositor

    Opher Gileadi

    Insert

    STX4 (RUNX1T1 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFLAG GLTPD2

    Plasmid

    #170745

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Insert

    GLTPD2 (GLTPD2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    V49F, V152A and D209E- Please see depositor comme…

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. HCP4

    Plasmid

    #166106

    Purpose

    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

    Depositor

    Matthias Heinemann

    Insert

    Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNSU299_RPL39

    Plasmid

    #166078

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    Tet-inducible

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. HCP6

    Plasmid

    #166108

    Purpose

    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

    Depositor

    Matthias Heinemann

    Insert

    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNSU299_YDR344C

    Plasmid

    #166072

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    Tet-inducible

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Z-CARS-8

    Plasmid

    #166555

    Purpose

    scFv of a human scaffold targeting Cysteinyl-tRNA synthetase. Antigen coverage aa 1-748 of 748, full-length

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Q-JMJD2DA-4

    Plasmid

    #166553

    Purpose

    scFv of a human scaffold targeting Lysine-specific demethylase 4D. Antigen coverage aa 1-342 of 523

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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