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pCS2plus-mCerulean 156-239-GGGS-TDP-43

Plasmid

#162617

Purpose

Allows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS-TDP-43

Plasmid

#162614

Purpose

Allows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS-TDP43 M337V

Plasmid

#162612

Purpose

Allows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

M337V

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP15 C-V5-tag

Plasmid

#179964

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp15, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 Nsp15 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 NSP1 C-V5-tag

Plasmid

#179982

Purpose

Mammalian expression vector for RaTG13 Nsp1, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

RaTG13 Nsp1

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 NSP1 C-V5-tag

Plasmid

#179972

Purpose

Mammalian expression vector for SARS-CoV-2 Nsp1, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-2 Nsp1 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V

Plasmid

#162615

Purpose

Allows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

M337V

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

Plasmid

#75126

Purpose

Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Mutation

SET domain replaced with the SET domain of EZH1

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pCMV_SEPT2 NCmut-msfGFP

Plasmid

#180320

Purpose

mammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

Plasmid

#75123

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

3x FLAG-tag

Mutation

bromodomain replaced with the first bromodomain o…

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pCS2plus-mVenus1-155-GGGS-TDP-43

Plasmid

#162611

Purpose

Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP1 C-V5-tag

Plasmid

#179961

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp1, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 Nsp1 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 NSP15 C-V5-tag

Plasmid

#179984

Purpose

Mammalian expression vector for RaTG13 Nsp15, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

RaTG13 Nsp15

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 M C-V5-tag

Plasmid

#179967

Purpose

Mammalian expression vector for SARS-CoV-1 M, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 M (M Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PARK7WT-VA

Plasmid

#115182

Purpose

Lentiviral transduction and expression of PARK7WT into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7WT (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-TARDBP-G368A

Plasmid

#141325

Purpose

Gateway cloning of TARDBP-G368A

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G368A (TARDBP Human)

Use

Gateway cloning

Mutation

G368A

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-TARDBP-W385G

Plasmid

#141326

Purpose

Gateway cloning of TARDBP-W385G

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-W385G (TARDBP Human)

Use

Gateway cloning

Mutation

W385G

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-TARDBP-G287A

Plasmid

#141323

Purpose

Gateway cloning of TARDBP-G287A

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G287A (TARDBP Human)

Use

Gateway cloning

Mutation

G287A

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPHAGE-C20orf24- C20orf24-3’UTR-WT

Plasmid

#128508

Purpose

Lentiviral constitutive expression of C20orf24 under control of its native WT 3'UTR of human C20orf24.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

RAB5IF (RAB5IF Human)

Use

Lentiviral

Tags

FLAG and HA tags

Expression

Mammalian

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3 Gmut-msfGFP

Plasmid

#180326

Purpose

mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i3 W502A, H512D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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