We narrowed to 7,126 results for: ef1a

1. pONSY-Lifeact:mCherry

   Plasmid

   #111876

   Purpose

   This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Lifeact fused to mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCh-466

   Plasmid

   #92251

   Purpose

   Plasmid for expression of GST-tagged human TNRC6C CED in mammalian cells

   Depositor

   Witold Filipowicz, Marina Chekulaeva

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690

   Promoter

   EF1A

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_103

   Plasmid

   #133460

   Purpose

   H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE8e SpG Puro

   Plasmid

   #235044

   Purpose

   Adenosine base editor 8e* with SpG nicking Cas9 makes A > G edits

   Depositor

   Justin Pritchard

   Article

   Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

   Insert

   ABE8e, nCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-PuroR

   Expression

   Mutation

   Promoter

   EF1a core

   Available since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-EFS-MCP-3XBFP-NLS

   Plasmid

   #236372

   Purpose

   Overexpression of MCP-3XBFPnls in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   MCP-3XBFPnls
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. EFS-mRuby2(miRE.FF4)-TS.FF6x1-bGH

   Plasmid

   #235309

   Purpose

   ComMAND open-loop circuit regulating mRuby2, EFS-driven

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS (human EF1a short, intronless)

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF.DEST51-Dok1

   Plasmid

   #215458

   Purpose

   Expression vector with Dok1

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   DOK1 (DOK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. EFS-mRuby2(miRE.FF4)-TS.FF4x1-bGH

   Plasmid

   #235310

   Purpose

   ComMAND closed-loop circuit regulating mRuby2, EFS-driven

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS (human EF1a short, intronless)

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. JD226

   Plasmid

   #229484

   Purpose

   Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k genes

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   E2A, VA RNA I, L4-22k
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (E2A), Ef1a (L4-22k) and wt promoter (VA RNA …

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK1302-AAV-EFSNC-dCjCas9-KRAB

    Plasmid

    #223147

    Purpose

    Expression of KRAB with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBK1312-AAV-EFSNC-dSaCas9-KRAB

    Plasmid

    #223157

    Purpose

    Expression of KRAB with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS-HsProm1s1(W795R)-StayGold

    Plasmid

    #211334

    Purpose

    Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    AAV

    Tags

    StayGold

    Expression

    Mutation

    Changed Trp-795 to Arg

    Promoter

    EF1a

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

    Plasmid

    #207171

    Purpose

    expression plasmid for Gal4DBD-HIF-CAD.myc expression

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-HIF1a-CAD Myc tag linker
    

    Use

    Tags

    2xMyc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-Puro_CI-hCOL4A5-Nluc_R1683X

    Plasmid

    #198111

    Purpose

    lentiviral expression of hCOL4A4_R1683Xin mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Nluc

    Expression

    Mutation

    R1683X

    Promoter

    EF1a

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-Puro_CI-hCOL4A5-Nluc_C1684X

    Plasmid

    #198112

    Purpose

    lentiviral expression of hCOL4A4_C1684X in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Nluc

    Expression

    Mutation

    C1684X

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-Puro_CI-hCOL4A5-Nluc_C29X

    Plasmid

    #198107

    Purpose

    lentiviral expression of hCOL4A5_C29X in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Nluc

    Expression

    Mutation

    C29X

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-Puro_CI-hCOL4A5-Nluc_R1563X

    Plasmid

    #198109

    Purpose

    lentiviral expression of hCOL4A4_R1563Xin mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Nluc

    Expression

    Mutation

    R1563X

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-Puro_CI-hCOL4A5-Nluc_S36X

    Plasmid

    #198108

    Purpose

    lentiviral expression of hCOL4A4_S36X in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Nluc

    Expression

    Mutation

    S36X

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-Puro_CI-hCOL4A5-Nluc

    Plasmid

    #198106

    Purpose

    lentiviral expression of hCOL4A4 in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Nluc

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L1-AA027

    Plasmid

    #185752

    Purpose

    Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Insert

    HygR2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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