We narrowed to 18,979 results for: INO
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Plasmid#222404Purposearabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.DepositorInsert5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
UseTags1xMS2 hairpin, 7GC clamp, TtrpA terminatorExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-6
Plasmid#227665PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Gm) used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionBacterialMutationPromoterp14G-BCD2Available sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-4
Plasmid#227664PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Sm) used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionBacterialMutationPromoterp14G-BCD2Available sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-2
Plasmid#227663PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionBacterialMutationPromoterp14G-BCD2Available sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNC-6
Plasmid#227669PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
UseTagsExpressionBacterialMutationPromoterPEM7Available sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNC-4
Plasmid#227668PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. SmR, easily curable via sucrose counterselection.DepositorInsertSmR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
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FAM177A1-SNAP
Plasmid#224592PurposeFor expression of FAM177A1-SNAPTag in mammalian cellsDepositorInsertFAM177A1 (FAM177A1 Human)
UseTagsSNAPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFlhb-t2a-GFP
Plasmid#194357Purposeover expression vector for killifish lhb tagged with GFPDepositorInsertluteinizing hormone subunit beta
UseLentiviralTagst2a-GFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV hGRN2
Plasmid#213683PurposeExpresses human granulin-2 with an N-terminal twin-Strep-FLAG tagDepositorInserthuman Granulin-2
UseAAVTagstwin-Strep-FLAGExpressionMutationPromotercytomegalovirus enhancer/chicken β-actin promoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV GFP
Plasmid#213685PurposeExpresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tagDepositorInsertTwin-Strep GFP
UseAAVTagsTwin-StrepExpressionMutationPromotercytomegalovirus enhancer/chicken β-actin promoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_EGR1p-BC1484-luc2
Plasmid#227138PurposeBarcoded assay, MAPK sensor; barcode BC1484DepositorInsertEGR1 promoter driving barcode 1484 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_EGR1p-BC0136-luc2
Plasmid#227137PurposeBarcoded assay, MAPK sensor; barcode BC0136DepositorInsertEGR1 promoter driving barcode 0136 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AS1371D
Plasmid#224417PurposeMammalian expression of KIF 13a S1371DDepositorInsertKIF 13a S1371D (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationS1371DPromoterChicken Beta actinAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MfnG-EcYRS-EGFP*
Plasmid#191119PurposeA plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafishDepositorInsertMfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHsp70(K71E)-GFP
Plasmid#228196Purposeexpressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tagDepositorInsertHSP70(K71E) (HSPA1A Human)
UseTagsGFPExpressionMammalianMutationK71EPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_EGR1p-BC0132-luc2
Plasmid#227136PurposeBarcoded assay, MAPK sensor; barcode BC0132DepositorInsertEGR1 promoter driving barcode 0132 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas3-Apr
Plasmid#208000PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ApyADO
Plasmid#226248PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyODepositorInsertsHis6_ApyA
ApyD
ApyO
UseTagsHexa-HistidineExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ApyAO
Plasmid#226242PurposeProduction of ApyA peptide modified by the cytochrome P450 enzyme ApyODepositorInsertsHis6_ApyA
ApyO
UseTagsHexa-HistidineExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ApyAD
Plasmid#226241PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyDDepositorInsertsHis6_ApyA
ApyD
UseTagsHexa-HistidineExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-WT
Plasmid#215897PurposeExpression of TNRC6A-silencing domain (SD)DepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1709PromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT
Plasmid#215898PurposeExpression of TNRC6A-CIM1 domainDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528PromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT
Plasmid#215899PurposeExpression of TNRC6A-CIM2 domainDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1529-1709PromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A
Plasmid#215900PurposeExpression of TNRC6A-CIM1 domain with S1332A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528, S1332APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A
Plasmid#215901PurposeExpression of TNRC6A-CIM1 domain with S1346A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1528, S1346APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AS1371A
Plasmid#224409PurposeMammalian expression of KIF 13a S1371ADepositorInsertKIF 13a S1371A (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationS1371APromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AWLDLE
Plasmid#224410PurposeMammalian expression of KIF 13a WLDLEDepositorInsertKIF 13a WLDLE (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationWLDLEPromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2111-FKBP-GPA-20-11S
Plasmid#222882PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFKBP-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-PD-L2-His10
Plasmid#223578PurposeProtein purification of human PD-L2 with SNAP and 10xHis tags in mammalian cellsDepositorInsertPD-L2 (PDCD1LG2 Human)
UseTagsHis10 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2375-FKBP-GPA-20-11S
Plasmid#222897PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFKBP-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2376-FKBP-GPA-20-114
Plasmid#222896PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFKBP-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2374-FRB-GPA-20-11S
Plasmid#222895PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFRB-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2373-FRB-GPA-20-114
Plasmid#222894PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFRB-GPA-20GS-NanoLuc114
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2179-FRB-GPA-6-GFPS
Plasmid#222893PurposeBiosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small.DepositorInsertFRB-GPA-6GS-GFPS
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2110-FKBP-GPA-20-114
Plasmid#222881PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFKBP-GPA-20GS-NanoLuc114
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2108-FRB-GPA-20-114
Plasmid#222879PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFRB-GPA-20GS-NanoLuc114
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2109-FRB-GPA-20-11S
Plasmid#222880PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFRB-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR101
Plasmid#185046PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with amino acids 2-8 deleted, trrnBDepositorInsertL31p
UseTagsExpressionBacterialMutationAmino acids 2-8 deleted of L31p coding regionPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR111
Plasmid#185047PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with last 8 amino acids deleted, trrnBDepositorInsertL31p
UseTagsExpressionBacterialMutationLast 8 amino acids deleted of L31p coding regionPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Hbb
Plasmid#99693PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-Flag-Miwi-EGFP
Plasmid#212575PurposeLentiviral expression of Flag-tagged mouse Miwi in mammalian cellsDepositorInsertMiwi (Piwil1 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA181
Plasmid#216024PurposeFragmid fragment: (Cas protein) unmodified AsCas12a; not preferredDepositorHas ServiceCloning Grade DNAInsertCas12a_v1.1 [As]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA016
Plasmid#215927PurposeFragmid fragment: (guide cassette) guide expressionDepositorHas ServiceCloning Grade DNAInsertU6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA048
Plasmid#216016PurposeFragmid fragment: (Cas protein) deactivated CasDepositorHas ServiceCloning Grade DNAInsertdCas12a (D908A)_v1.1 [EnAs]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_816
Plasmid#216098PurposeCRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)DepositorInsertCas12a [EnAs]
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-OEP7-mVenus
Plasmid#213864PurposeBinary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.DepositorInsertchloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_04
Plasmid#194665PurposeProtein production of the split HaloTag based calcium recorder Caprola_04 in bacteriaDepositorInsertCaprola_04
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_06
Plasmid#194667PurposeProtein production of the split HaloTag based calcium recorder Caprola_06 in bacteriaDepositorInsertCaprola_06
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_07
Plasmid#194668PurposeProtein production of the split HaloTag based calcium recorder Caprola_07 in bacteriaDepositorInsertCaprola_07
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only