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pLenti-TRE3G-BE3-PGK-Puro

Plasmid

#110845

Purpose

Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

TRE3G

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLD1_2

Plasmid

#160792

Purpose

Suppress POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLD1_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-C91G-GFP

Plasmid

#78916

Purpose

Mammalian expression of BAP1 with C91G and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

C91G

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro

Plasmid

#118702

Purpose

lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

wt , SLC7A11 sh926R

Promoter

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3T911A

Plasmid

#120254

Purpose

Expression of ATP3-6 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

T911 changed to A; Isoleucine 304 changed to Aspa…

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-S63C-GFP

Plasmid

#78911

Purpose

Mammalian expression of BAP1 with S63E and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

S63C

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pLKO.1P POLE1_2

Plasmid

#160764

Purpose

Suppress POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pX330 NL-FKBP-Cas9

Plasmid

#162723

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5

Plasmid

#112840

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Full length Grb2

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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LightR-bRaf-mVenus

Plasmid

#162154

Purpose

Expresses LightR-bRaf-mVenus in mammalian cells

Depositor

Andrei Karginov

Article

Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

Insert

LightR-bRaf-mVenus

Use

Tags

mVenus

Expression

Mammalian

Mutation

Valine 600 is substituted with Glutamic acid

Promoter

CMV

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-ERR3 S57,81,219A

Plasmid

#37850

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

Estrogen-related receptor gamma (Esrrg Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Serines 57, 81, and 219 to Alanine (AGC t…

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
pENTR4 PARP2 E614Q

Plasmid

#192545

Purpose

pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)

Use

Gateway entry vector

Tags

Expression

Mutation

changed Glu 614 to Gln

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBabepuro3-p18

Plasmid

#24935

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p18 (CDKN2C Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-BE3RA-P2A-Puro

Plasmid

#110839

Purpose

Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pX330 N-FKBP-Cas9

Plasmid

#162727

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DDX11_2

Plasmid

#160778

Purpose

Suppress DDX11

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDDX11_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DDX11_1

Plasmid

#160777

Purpose

Suppress DDX11

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDDX11_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-mNeonGreen

Plasmid

#205017

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

PpsbA

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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CK2alpha V66A/I174A-HA (pJD23)

Plasmid

#37362

Depositor

David Litchfield

Article

Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

Insert

CK2α V66A/I174A (CSNK2A1 Human)

Use

Tags

HA X3

Expression

Mammalian

Mutation

V66A, I174A

Promoter

CMV

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
sgRNA BRCA1 E638K

Plasmid

#139327

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 E638K using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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