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pActinin-miRFP703

Plasmid

#79992

Purpose

Alpha actinin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Alpha-Actinin (ACTN1 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

c

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCB6-Cor1-HA

Plasmid

#102815

Purpose

Expression of Coronin1 in eukaryotic cells

Depositor

Jean Pieters

Article

Gatfield et al Mol Biol Cell. 2005 Jun;16(6):2786-98. E

Insert

TACO/Coronin 1 (Coro1a Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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CS2-Myc-hASUN

Plasmid

#47955

Purpose

Expression of tagged-human ASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2013 Jul 31.

Insert

Asunder (INTS13 Human)

Tags

Myc

Expression

Mammalian

Promoter

Sp6

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pDest-636

Plasmid

#159574

Purpose

Gateway Destination vector for protein expression in baculovirus

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Type

Empty backbone

Tags

His6-MBP

Expression

Insect

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE Flag HA ADAR2 CI

Plasmid

#237997

Purpose

Inducible lentiviral expression of ADAR2 CI

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

ADAR2 (ADARB1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E396A

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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Psicheck2 COLIA1 full length 3'UTR

Plasmid

#26993

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

COL1A1 Full length 3' UTR (COL1A1 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pLdCH

Plasmid

#84291

Purpose

Express gRNA and Cas9 in Leishmania with Hygromycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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dCas9-NLS-gs10-cTRb413

Plasmid

#188261

Purpose

Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

cTRb413 (TXNRD2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.T7 delta-NFAT3

Plasmid

#28224

Depositor

Sebastien Jauliac

Article

Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

Insert

Delta NFAT3 (NFATC4 Human)

Tags

T7

Expression

Mammalian

Mutation

hNFAT3 WT deleted amino acids 1-342

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pII pm

Plasmid

#218099

Purpose

Phagemid that expresses LacI, and pII under the Lac promoter

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Insert

pII

Expression

Bacterial

Promoter

Lac promoter

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD-IFP2.0C

Plasmid

#215750

Purpose

Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD_W42A-IFP2.0C

Plasmid

#215751

Purpose

Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD_W42A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pVimentin-miRFP703

Plasmid

#79996

Purpose

Vimentin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Vimentin (VIM Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-Strep/HA

Plasmid

#124666

Purpose

Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE puro Brca1 S1423A S1524A HA

Plasmid

#41968

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

BRCA1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

S1423A, S1524A

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pRSpuro shID1#6

Plasmid

#19165

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 1 (ID1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

Plasmid

#129513

Purpose

Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 C88S (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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LightR-iCre-miRFP670

Plasmid

#162158

Purpose

Expresses LightR-iCre-miRFP670 in mammalian cells

Depositor

Andrei Karginov

Article

Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

Insert

LightR-iCre-miRFP670

Tags

NLS and miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEND-193_Cacnes_HS

Plasmid

#225622

Purpose

C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

HspR

Use

Synthetic Biology

Tags

pFAST-6XHis

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-192_Cacnes_HS

Plasmid

#225621

Purpose

C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

HspR

Use

Synthetic Biology

Tags

pFAST-6XHis

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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