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pEh_Cas5

Plasmid

#178755

Purpose

Expression of E. haloalkaliphila type I-E cas5

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

E. haloalkaliphila type I-E cas5

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc2_Cas8

Plasmid

#178735

Purpose

Expression of A. chroococcum type I-E #2 cas8

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

A. chroococcum type I-E #2 cas8

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc2_Cse2

Plasmid

#178736

Purpose

Expression of A. chroococcum type I-E #2 cse2

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

A. chroococcum type I-E #2 cse2

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc2_Cas5

Plasmid

#178737

Purpose

Expression of A. chroococcum type I-E #2 cas5

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

A. chroococcum type I-E #2 cas5

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc2_Cas6

Plasmid

#178738

Purpose

Expression of A. chroococcum type I-E #2 cas6

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

A. chroococcum type I-E #2 cas6

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc2_Cas7

Plasmid

#178739

Purpose

Expression of A. chroococcum type I-E #2 cas7

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

A. chroococcum type I-E #2 cas7

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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NK651

Plasmid

#109107

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_07215 deltaCC

Use

Choanoflagellate expression

Mutation

Deletes C-terminal coiled-coil from PTSG_07215

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK617

Plasmid

#109095

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

S. cerevisiae CoxIV target sequence

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Rab33bb cds in pGem-T Easy

Plasmid

#80524

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab33bb

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pTCW010

Plasmid

#83521

Purpose

Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha2 gene

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pARO9-mfalpha2

Expression

Yeast

Promoter

pARO9

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTCW009

Plasmid

#83523

Purpose

Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha1 gene

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pARO9-mfalpha1

Expression

Yeast

Promoter

pARO9

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTCW005

Plasmid

#83524

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-malpha1

Expression

Yeast

Promoter

pFUS1

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pNB767

Plasmid

#60159

Purpose

Firefly luciferase (Promega) driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB649

Plasmid

#60136

Purpose

Firefly luciferase (Promega) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-hTERT-IRES-hygro

Plasmid

#85140

Purpose

Lentiviral expression of hTERT, used to create immortalized cell lines

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

hTERT (TERT Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Article

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132398

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Alpha 5 integrin-GFP

Plasmid

#15238

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

alpha 5 integrin (ITGA5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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