We narrowed to 63,501 results for: cell

1. NK651

   Plasmid

   #109107

   Purpose

   Reporter gene expression in S. rosetta

   Depositor

   Nicole King

   Article

   Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

   Insert

   PTSG_07215 deltaCC
   

   Use

   Choanoflagellate expression

   Tags

   Expression

   Mutation

   Deletes C-terminal coiled-coil from PTSG_07215

   Promoter

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. NK617

   Plasmid

   #109095

   Purpose

   Reporter gene expression in S. rosetta

   Depositor

   Nicole King

   Article

   Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

   Insert

   S. cerevisiae CoxIV target sequence
   

   Use

   Choanoflagellate expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rab33bb cds in pGem-T Easy

   Plasmid

   #80524

   Purpose

   Can be used for in situ hybridisation or other purposes

   Depositor

   Rob Parton

   Article

   Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

   Insert

   rab33bb
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTCW010

   Plasmid

   #83521

   Purpose

   Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha2 gene

   Depositor

   Claudia Vickers

   Article

   Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

   Insert

   pARO9-mfalpha2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pARO9

   Available Since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTCW009

   Plasmid

   #83523

   Purpose

   Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha1 gene

   Depositor

   Claudia Vickers

   Article

   Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

   Insert

   pARO9-mfalpha1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pARO9

   Available Since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTCW005

   Plasmid

   #83524

   Purpose

   pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene

   Depositor

   Claudia Vickers

   Article

   Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

   Insert

   pFUS1-malpha1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pFUS1

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB767

   Plasmid

   #60159

   Purpose

   Firefly luciferase (Promega) driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   A4V & S504G (no functional effect)

   Promoter

   SIC1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNB649

   Plasmid

   #60136

   Purpose

   Firefly luciferase (Promega) driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-hTERT-IRES-hygro

   Plasmid

   #85140

   Purpose

   Lentiviral expression of hTERT, used to create immortalized cell lines

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

    Plasmid

    #132404

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDM304_DPAKa(GBD)-YFP

    Plasmid

    #128699

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Expression

    Mutation

    none

    Promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132397

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1058A

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

    Plasmid

    #132398

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132400

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Promoter

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132409

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRanCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H147A; H1044A

    Promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid

    #74707

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-Puro CAG FUCCI

    Plasmid

    #136934

    Purpose

    donor plasmid for constitutive FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
    

    Use

    Synthetic Biology

    Tags

    Clover and mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. tetO7_Ef1a_SOX2

    Plasmid

    #18919

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

20. PGK1p-Csy4-pA (Construct 2)

    Plasmid

    #55196

    Purpose

    Expresses Csy4 in Mammalian cells for site-specific RNA processing

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    Csy4/Cas6
    

    Use

    Synthetic Biology

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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