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NK617

Plasmid

#109095

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

S. cerevisiae CoxIV target sequence

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Rab33bb cds in pGem-T Easy

Plasmid

#80524

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab33bb

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pTCW005

Plasmid

#83524

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-malpha1

Expression

Yeast

Promoter

pFUS1

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pNB767

Plasmid

#60159

Purpose

Firefly luciferase (Promega) driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB649

Plasmid

#60136

Purpose

Firefly luciferase (Promega) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-hTERT-IRES-hygro

Plasmid

#85140

Purpose

Lentiviral expression of hTERT, used to create immortalized cell lines

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

hTERT (TERT Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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GIT1A-FLAG

Plasmid

#15225

Depositor

Rick Horwitz

Article

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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Alpha 5 integrin-GFP

Plasmid

#15238

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

alpha 5 integrin (ITGA5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Article

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-neo largeTcDNA

Plasmid

#1780

Purpose

Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132398

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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mTiam1-mcherry-sspB in pcDNA3.1

Plasmid

#85221

Purpose

Role of Rac selective GEF domain from Tiam1 in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

mTiam1 (1030-1406) (Tiam1 Mouse)
mcherry
sspB

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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CB6-GFP-TAOK2 (K57A)

Plasmid

#197114

Purpose

Expression of kinase-deficient GFP-tagged TAOK2 (K57A) / PSK1 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK2 (K57A) (TAOK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

CMV

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

Plasmid

#132404

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-hOCT3/4

Plasmid

#17217

Purpose

Retroviral expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

OCT3/4 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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PR_GEF YFP (2XPDZ-YFP-LARG DH)

Plasmid

#80408

Purpose

Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCGN-HCF-1-N1011

Plasmid

#92320

Purpose

N-term 1-1011 of HCF-1 protein

Depositor

Winship Herr

Article

Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

Insert

HCF-1 (HCFC1 Human)

Tags

HA

Expression

Mammalian

Mutation

AA 2-1011

Promoter

CMV

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

Plasmid

#132407

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLwaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R474A; H479A; R1046A; R1051A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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