We narrowed to 3,095 results for: sam

1. pCag FlpO-2A-Cre EV

   Plasmid

   #129419

   Purpose

   episomal expression of FlpO and Cre recombinases

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Expression

   Mammalian

   Promoter

   CMV/Chick β-actin (CAG)

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYX-C-mScarlet-Zeo-TSC22D2

   Plasmid

   #222670

   Purpose

   endogenously tag TSC22D2 with C-terminal mScarlet

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D2 (TSC22D2 Human)
   

   Tags

   mScarlet

   Expression

   Bacterial

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO-PPO-Venus

   Plasmid

   #139505

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV8 and AAV9

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR 223 VPS4A

   Plasmid

   #169319

   Purpose

   Gateway entry vector for human VPS4A ORF

   Depositor

   Francisca Vazquez

   Article

   Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

   Insert

   VPS4A (VPS4A Human)
   

   Use

   Gateway entry vector

   Promoter

   NA

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AIP(WT)-mChF-giantin

   Plasmid

   #61523

   Purpose

   Expression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Inserts

   FK506 binding protein 1A (FKBP1A Human)
   golgin B1 (GOLGB1 Human)
   

   Tags

   AIP and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCAGGS/XBB.1.16-RBD

   Plasmid

   #213070

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/XBB.1.16-RBD (S )
   

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZ:F3-CAGGS GPHTK-F

   Plasmid

   #112666

   Purpose

   Genome engineering donor vector for creating master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus

   Depositor

   Catherine Verfaillie

   Article

   Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

   Insert

   eGFP
   

   Use

   Gene targeting vector for integration at the s1 s…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pαH-S-GSAS-2P-Omicron-BA.5

   Plasmid

   #213828

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pαH-S-GSAS/Omicron-2P-BA5 (S )
   

   Tags

   HRV 3C cleavage site (before tags) 8X His tag 2X…

   Expression

   Mammalian

   Mutation

   G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223 SARS-CoV-2 NSP6_nostop

   Plasmid

   #149309

   Purpose

   Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid

    #112668

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-DIO-PPO-Venus

    Plasmid

    #139504

    Purpose

    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Has Service

    AAV5

    Insert

    parapinopsin
    

    Use

    AAV

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR 223 VPS4B

    Plasmid

    #169320

    Purpose

    Gateway entry vector for human VPS4B ORF

    Depositor

    Francisca Vazquez

    Article

    Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

    Insert

    VPS4B (VPS4B Human)
    

    Use

    Gateway entry vector

    Promoter

    NA

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYX-N-Puro-mNeon-WNK3

    Plasmid

    #222674

    Purpose

    endogenously tag WNK3 with N-terminal mNeon

    Depositor

    Jason Moffat

    Article

    Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

    Insert

    WNK3 (WNK3 Human)
    

    Tags

    mNeon

    Expression

    Bacterial

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223 SARS-CoV-2 NSP5_nostop

    Plasmid

    #149308

    Purpose

    Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pPICZc-HsACTB*-thymosinB-8His

    Plasmid

    #111146

    Purpose

    Expresses human beta-actin fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    ACTB (ACTB Human)
    

    Use

    Pichia pastoris integration

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. BCL6-GFP G55A in Artichoke

    Plasmid

    #169935

    Purpose

    Overexpression of BCL6-GFP G55A mutant fusion

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

    Insert

    BCL6 G55A (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Mutation

    160R>C

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid

    #111147

    Purpose

    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    actin gamma 1 (ACTG1 Human)
    

    Use

    Pichia pastoris integration vector

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    codon-optimised for expression in Pichia pastoris

    Promoter

    AOX1

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid

    #185706

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    Cas9
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZ:F3-P CAGGS tdTPH-F

    Plasmid

    #112667

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223 SARS-CoV-2 NSP9_nostop

    Plasmid

    #149312

    Purpose

    Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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