We narrowed to 2,609 results for: ubiquitin

1. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

   Plasmid

   #183458

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

   Plasmid

   #169960

   Purpose

   Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG and Ub

   Expression

   Mammalian

   Mutation

   F370G

   Promoter

   CMV

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInducer10-UBC9-sh3

   Plasmid

   #168987

   Purpose

   Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Ubiquitin Conjugating Enzyme 9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G

   Plasmid

   #169959

   Purpose

   Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG and Ub

   Expression

   Mammalian

   Mutation

   L368G

   Promoter

   CMV

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E

   Plasmid

   #169961

   Purpose

   Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG and Ub

   Expression

   Mammalian

   Mutation

   R394E

   Promoter

   CMV

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G

   Plasmid

   #170119

   Purpose

   Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG and Ub

   Expression

   Mammalian

   Mutation

   F405G

   Promoter

   CMV

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

   Plasmid

   #58490

   Purpose

   Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

   Insert

   Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Aspartic Acid 95 to Glutamine

   Promoter

   Ubiquitin

   Available since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1-Flag-Rheb

   Plasmid

   #165031

   Purpose

   expression of Flag tagged Rheb protein in mammalian cells

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1-Flag-Rheb-4Rs

   Plasmid

   #165022

   Purpose

   expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb-4Rs (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   change lysine residues on position 109, 135, 151,…

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1-Flag-Rheb-12Rs

    Plasmid

    #165023

    Purpose

    expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb-12Rs (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    All the lysine residues except K19 and K120 are c…

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO/GFPZNF598-9P-9A

    Plasmid

    #141192

    Purpose

    Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    all 3 proline repeats are mutated to Alanine (9xP…

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH-nCas9-PPE-V2

    Plasmid

    #170131

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

    Insert

    nCas9(H840A)-M-MLV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A for Cas9; D200N, T306K, W313F, T330P and L…

    Promoter

    maize Ubiquitin-1, OsU3

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUGW-APPSwe-T2A-mCherry

    Plasmid

    #190804

    Purpose

    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Article

    Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Swedish (KM670/671NL)

    Promoter

    Ubi C

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P2-MDM2 (17-125)

    Plasmid

    #62063

    Purpose

    Expression of human MDM2 (17-125) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 17-125

    Promoter

    tac

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid

    #159107

    Purpose

    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

    Depositor

    Thomas Tuschl

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    NSP12
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-C-prM-E-NS1

    Plasmid

    #175281

    Purpose

    lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    C-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
    

    Use

    Lentiviral

    Tags

    IRES EGFP

    Expression

    Mutation

    Promoter

    human ubiquitin C

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2K7bsdUBI-mCherry-STIM1

    Plasmid

    #114178

    Purpose

    Lentiviral vector for expression of mCherry-STIM1

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry (inserted after signal peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTol2CG2-ubi:QFGal4-SV40pA

    Plasmid

    #155119

    Purpose

    Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    ubi:QFGal4 (ubb Neurospora crassa, Zebrafish, Budding Yeast)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    ubiquitin B

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid

    #178057

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-FLAG
    

    Use

    AAV

    Tags

    FLAG-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX6P2-MDMX (23-111)

    Plasmid

    #62064

    Purpose

    Expression of human MDM4 (residues 23-111) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 23-111

    Promoter

    tac

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only