We narrowed to 7,360 results for: ALP

1. APEX2 - Tubulin in pEGFP

   Plasmid

   #66171

   Purpose

   APEX2 fused to the N-terminus of alpha tubulin

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 - alpha tubulin
   

   Use

   Tags

   Flag epitope tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   APEX2 contains 4 mutations relative to wt soybean…

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. FnR9-10

   Plasmid

   #182567

   Purpose

   Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 9-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP Tubulin K40R

   Plasmid

   #105303

   Purpose

   To study effects of acetylation-null tubulin

   Depositor

   Kenneth Yamada

   Article

   Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

   Insert

   alpha tubulin (TUBA1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K40R

   Promoter

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-surfaceHA-IRES-Puro

   Plasmid

   #207321

   Purpose

   Lentiviral expression of synthetic HA surface receptor in Mammalian cells

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   HAtag-Myctag-PDGFRB-TM-IRES-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha_short (EFS)

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-6P-1 Stu2 1-590

   Plasmid

   #38314

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVET-tTR-KRAB

   Plasmid

   #11644

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hEF-1alpha, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGas-miRFP670nano

   Plasmid

   #127441

   Purpose

   G protein alpha-s internally tagged with miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   G protein alpha-s-miRFP670nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. YFP-DAT

   Plasmid

   #90228

   Purpose

   Expresses human DAT in mammalian cells

   Depositor

   Alexander Sorkin

   Article

   Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-8

   Insert

   DAT (dopamine transporter) (SLC6A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTubulin-miRFP704nano

   Plasmid

   #206192

   Purpose

   Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP704nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

   Insert

   miRFP704nano-human alpha-tubulin
   

   Use

   Tags

   miRFP704nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAS-syb:spGFP1–10

    Plasmid

    #73698

    Purpose

    Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

    Depositor

    Marco Gallio

    Article

    Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

    Insert

    Neuronal-synaptobrevin:spGFP1–10 chimera
    

    Use

    Drosophila

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIG3 B

    Plasmid

    #132942

    Purpose

    MLV packaging plasmid for B-tropic MLV gag-pol expression

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    B-tropic MLV gag-pol
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry-C1-FAK-HA

    Plasmid

    #35039

    Depositor

    Anna Huttenlocher

    Article

    Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

    Insert

    focal adhesion kinase (Ptk2 Mouse)
    

    Use

    Tags

    2x HA and mCherry

    Expression

    Mammalian

    Mutation

    Y42H; deletion of amino acids 1051 & 1052

    Promoter

    Available since

    March 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. FR_HNF6

    Plasmid

    #31099

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF6 (ONECUT1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSIL-eGFP

    Plasmid

    #52675

    Purpose

    shRNA silencing plasmid with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Type

    Empty backbone

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    CMV and U6

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. psd44-G12QL

    Plasmid

    #46825

    Purpose

    expression of mutant G protein alpha 12 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA12 (GNA12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q231L (the mutation reduces GTPase activity resul…

    Promoter

    PGK

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. KG#62

    Plasmid

    #110874

    Purpose

    Expresses the C. elegans acy-1 cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

    Inserts

    rab-3 promoter
    acy-1 cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-PIK3R1

    Plasmid

    #174191

    Purpose

    Expresses PIK3R1 in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCE-P2A-Puro

    Plasmid

    #123318

    Purpose

    Lentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCG-Hc∆18-AA-IL-13

    Plasmid

    #86171

    Purpose

    Plasmid encodes blinded measles virus H glycoprotein displaying IL-13

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Measles virus H glycoprotein fused to IL-13
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    Y463A, R515A

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pECFP-histone H3

    Plasmid

    #179698

    Purpose

    visualization of nucleus/chromosome

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    Histone H3 (Hist1h3h Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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