We narrowed to 18,240 results for: gateway

1. IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

   Plasmid

   #121826

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/Dnmt3a-3L (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. KO602: pMVP (L3-L2) HA tag + pA; CMV::TETa-pA

   Plasmid

   #121802

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + CMV::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

   Plasmid

   #121803

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + EF1a::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

   Plasmid

   #121807

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + RIP::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KO502: pMVP (L3-L2) HA tag + pA; RIP::TETa-pA

   Plasmid

   #121810

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + RIP::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

   Plasmid

   #121815

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter

   Plasmid

   #121818

   Purpose

   pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

   Plasmid

   #121820

   Purpose

   pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. JH204: pMVP (R4-R3) human PDX1 open

   Plasmid

   #121745

   Purpose

   pMVP R4-R3 entry plasmid, contains human PDX1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Human PDX1 (open) (PDX1 Human)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFOSGE_COF3H04

    Plasmid

    #70971

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf6 (Taf6 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pFOSGE_COF3C07

    Plasmid

    #70985

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    14-3-3zeta (14-3-3zeta Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pMuLE ENTR U6 n.s shRNA L1-L4

    Plasmid

    #62136

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    non-silencing (scrambled) shRNA
    

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuLE ENTR U6 n.s shRNA R4-R3

    Plasmid

    #62137

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    non-silencing (scrambled) shRNA
    

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMuLE ENTR U6 n.s shRNA L5-L2

    Plasmid

    #62139

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    non-silencing (scrambled) shRNA
    

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMuLE ENTR 7SK-miR-30 R4-R3

    Plasmid

    #62121

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMuLE ENTR 7SK-miR-30 L1-R5

    Plasmid

    #62118

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDEST26-NAT1-C-HA

    Plasmid

    #195178

    Purpose

    Vector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

    Depositor

    Stephanie Roessler

    Insert

    NAT1 (NAT1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONRCBX

    Plasmid

    #29634

    Depositor

    Scott Gold, David_L Andrews

    Article

    Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

    Type

    Empty backbone

    Use

    Gateway donor

    Available Since

    Sept. 19, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGEX6P1-DEST-His

    Plasmid

    #119763

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Tags

    10xHis and GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 373 pTol2-fli1ep:CherryDest

    Plasmid

    #73493

    Purpose

    Tol2 vector with fli1ep element upstream of mCherry, attR1/R2 cassette, and late SV40 pA signal

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    fli1ep:mCherry-attR1-attR2
    

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pQLinkHD

    Plasmid

    #13668

    Depositor

    Konrad Buessow

    Article

    Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

    Insert

    Gateway cassette
    

    Use

    Co-expression

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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22. pQLinkGD

    Plasmid

    #13673

    Depositor

    Konrad Buessow

    Article

    Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

    Insert

    Gateway cassette
    

    Use

    Co-expression

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 372 pTol2-fli1ep:EGFPDest

    Plasmid

    #73491

    Purpose

    Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signal

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    fli1ep:EGFP-attR1-attR2
    

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGGDestSC+ATG

    Plasmid

    #49323

    Purpose

    Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Destination vector

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pStart-K

    Plasmid

    #20346

    Depositor

    Mario Capecchi

    Article

    Wu et al Nat Protoc. 2008 . 3(6):1056-76.

    Type

    Empty backbone

    Use

    Mouse Targeting; Gateway cloning

    Available Since

    March 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. pENTR1a-jAspSnFR3

    Plasmid

    #203460

    Purpose

    jAspSnFR3 in Gateway cloning entry vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    jAspSnFR3
    

    Use

    Gateway entry vector

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX6P1-DEST-FLAG

    Plasmid

    #119754

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Tags

    FLAG and GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEG545

    Plasmid

    #40116

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Type

    Empty backbone

    Use

    Gateway donor vector

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Available Since

    Sept. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pEN12A-P1

    Plasmid

    #27366

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    Pmyc1tetO
    

    Use

    Gateway donor vector

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. pGEX6P1-DEST-Myc

    Plasmid

    #119752

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Tags

    GST and Myc

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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