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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 42,575 results for: cha;

Showing: 4681 - 4720 of 42575 results

  1. pJT1

    Plasmid
    #98299
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    98299-plasmid-map-sequence-id-189966

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  2. pFUGW-hGtACR2-EYFP

    Plasmid
    #67877
    Purpose
    Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone
    Depositor
    John Spudich
    Article
    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.
    Insert
    hGtACR2
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67877-plasmid-map-sequence-id-199004

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  3. pFUGW-hGtACR1-EYFP

    Plasmid
    #67795
    Purpose
    Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone
    Depositor
    John Spudich
    Article
    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.
    Insert
    hGtACR1
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67795-plasmid-map-sequence-id-120489

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  9. R57C10-Flp2

    Plasmid
    #63172
    Purpose
    R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion
    Depositor
    Gerald Rubin
    Article
    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p
    Insert
    yeast wild type containing a Glycine at amino acid 5 of the protein sequence
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pUDP293

    Plasmid
    #204228
    Purpose
    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA
    Depositor
    Jean-Marc Daran
    Article
    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d
    Inserts
    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mutation
    codon optimized for S. cerevisiae
    Promoter
    Saccharomyces cerevisiae PGK1 and Saccharomyces c…
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    204228-plasmid-map-sequence-id-408527

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  16. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

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  19. ZJOM153

    Plasmid
    #133642
    Purpose
    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    Vph1-2GFP (VPH1 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133642-plasmid-map-sequence-id-268960

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  20. ZJOM31

    Plasmid
    #133645
    Purpose
    Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-TGL3-GFP (TGL3 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133645-plasmid-map-sequence-id-265890

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  36. pHL 246

    Plasmid
    #53014
    Purpose
    AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    fimS (off orientation)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pPMVAu5 (R)

    Plasmid
    #98296
    Purpose
    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98296-plasmid-map-sequence-id-201300

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Showing: 4681 - 4720 of 42575 results