We narrowed to 8,780 results for: construct
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Plasmid#86455PurposeNuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
1203R-CMV-3'dmDR-EGFP-bGHpolyA
Plasmid#220962PurposeEGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 3' endDepositorInserteGFP
ExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203C-CMV-5’+3'DR-EGFP-bGHpolyA
Plasmid#221454PurposeEGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end and 3' endDepositorInserteGFP
ExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT2/shαSMA/GFP4_Seq2/clone1
Plasmid#201402PurposeKnockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertalpha-SMA
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Wheat_live_Cas9
Plasmid#126880PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-proD
Plasmid#65820PurposeConst. Promoter ProD targetting RNA E. coli Low Phosphate InductionDepositorInsertproD guide
PromoterE . coli ugpB gene promoter, low phosphate induc…Available SinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ROSAr
Plasmid#54324PurposeTALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmidDepositorInsertTALEN for the murine ROSA26 locus
UseMouse Targeting and TALENExpressionMammalianPromoterCAGGAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJ251-GERC
Plasmid#47441PurposeExpresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.DepositorInsertGene Expression Reporter Construct
ExpressionBacterialAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]
Plasmid#86453PurposeUAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)DepositorInsert3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
DH-BB(5'SIN;TE12ORF)
Plasmid#72309PurposeDefective Helper to produce neurotropic, propagation-incompetent Sindbis virusDepositorInsertDH-BB(5'SIN;TE12ORF)
UseSindbis virus helper constructAvailable SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
RARS_pNIC-Bio3
Plasmid#153055PurposeExpression construct for producing Avi tagged Arginyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
mem-mCherry-pTRIP
Plasmid#163521PurposeUsed to construct lentivirus to express membrane-targeted mCherry in mammalian cellsDepositorInsertmembrane targeted mCherry
UseLentiviralExpressionBacterial and MammalianAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
nuc-mCherry-pTRIP
Plasmid#163520PurposeUsed to construct lentivirus to express nucleus-targeted mCherry in mammalian cellsDepositorInsertnucleus targeted mCherry
UseLentiviralExpressionBacterial and MammalianAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
TARS_pNIC-Bio3
Plasmid#153057PurposeExpression construct for producing Avi tagged Threonyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-OPN-1200
Plasmid#11996DepositorInsertOsteopontin (SPP1 Human)
ExpressionMammalianMutationThe following construct contains the OPN promoter…Available SinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ROSAf
Plasmid#54323PurposeTALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmidDepositorInsertTALEN for the murine ROSA26 locus
UseMouse Targeting and TALENExpressionMammalianPromoterCAGGAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]
Plasmid#86454PurposeNuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
NARS_pNIC-Bio3
Plasmid#153054PurposeExpression construct for producing Avi tagged Asparaginyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-CRE-T2A-PuroR
Plasmid#172298PurposeIn vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettesDepositorInsertCRE-T2A-PuroR
UseCre/LoxPromoterT7 promoter A-initiatingAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNanog-Halo-Ty2-BactBsd
Plasmid#235511PurposeC-term HALO targeting constructDepositorAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203D-5'dDR-mCh(22)-GFP-bGHpolyA
Plasmid#221534PurposeEGFP reporter construct with double unprocessed direct repeat (35nt) at 5' end, 22nt mCherry-targeting spacer in between DRsDepositorInserteGFP
ExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203E-3'dDR-mCh(22)-GFP-bGHpolyA
Plasmid#221535PurposeEGFP reporter construct with double unprocessed direct repeat (35nt) at 3' end, 22nt mCherry-targeting spacer in between DRsDepositorInserteGFP
ExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203Q-5'dmDR-NT(22)-GFP-bGHpolyA
Plasmid#221538PurposeEGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' endDepositorInserteGFP
ExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203S-5'+3’dmDR-NT(22)-GFP-bGHpolyA
Plasmid#221539PurposeEGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' and 3' endDepositorInserteGFP
ExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
L0-T11 Circuit 2 GG4-PhiC31B-Bxb1B-GG6
Plasmid#219694PurposeLevel 0 to construct history-dependent target T2BPDepositorInsertGG4-PhiC31B-Bxb1B-GG6
UseSynthetic BiologyAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T12 GG2-ER-tagBFP-tUBQ10-GG3
Plasmid#219695PurposeLevel 0 to construct history-dependent targetDepositorInsertGG2-ER-tagBFP-tUBQ10-GG3
UseSynthetic BiologyAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK luciferase-V5 neo
Plasmid#220364PurposeLentiviral expression vector for an inducible V5-tagged luciferase constructDepositorInsertluciferase
UseLentiviralTagsV5ExpressionMammalianAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T13 GG3-tRBCS-mCherry-NLS-GG4
Plasmid#219696PurposeLevel 0 to construct history-dependent targetDepositorInsertGG3-tRBCS-mCherry-NLS-GG4
UseSynthetic BiologyAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1
Plasmid#219697PurposeLevel 0 to construct history-dependent targetDepositorInsertGG0-tUBQ1-NLS-NeonGreen-GG1
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T10 Circuit 1 GG4-Bxb1B-PhiC31B-GG6
Plasmid#219693PurposeLevel 0 to construct history-dependent target T2PBDepositorInsertGG4-Bxb1B-PhiC31B-GG6
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-CRE-T2A-BleoR
Plasmid#172300PurposeIn vitro transcription of CRE-T2A-BleoR synthetic construct for the excision of floxed cassettesDepositorInsertCRE-T2A-BleoR
UseCre/LoxPromoterT7 promoter A-initiatingAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_Rac5
Plasmid#126897PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRAvailable SinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ OBfold
Plasmid#126901PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ HDT701
Plasmid#126898PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTRY4_ ERF922
Plasmid#126893PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-zwf
Plasmid#65825Purposezwf targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertzwf guide
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induc…Available SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA
Plasmid#65818PurposeudhA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertudhA guide
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induc…Available SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2
Plasmid#65817PurposegltA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA2 guide
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induc…Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGD-p19
Plasmid#196326Purpose35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressorDepositorInserttomato bushy stunt virus p19
ExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ratiometric FPX sensor for ERK kinase activity
Plasmid#60974PurposeFor imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.DepositorInsertddRFP A-WW domain-ERK substrate-ddFP B
TagsERK substrate is before ddFP copy B, WW domain is…ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only