Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 13,335 results for: ache

Showing: 5851 - 5900 of 13335 results

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pET28b-NmCas9-Flag D16A

Plasmid

#109031

Purpose

D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-fsaA

Plasmid

#102286

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

fsaA gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-adhE

Plasmid

#102290

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

adhE gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ompT

Plasmid

#102291

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

ompT gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRRG312-slc1a-5

Plasmid

#99102

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea slc1a-5 (also known as excitatory amino acid transporter)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

slc1a-5 (also known as excitatory amino acid transporter) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG94-runt-1

Plasmid

#99089

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Runt-1 (also known as runt)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

Runt-1 (also known as runt) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-srlA

Plasmid

#89965

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

srlA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-rbsK

Plasmid

#89963

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rbsK gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-yhhT

Plasmid

#89958

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

yhhT gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-torZ

Plasmid

#89956

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

torZ gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-arsB

Plasmid

#89952

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

arsB gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSI HA Nup53xl

Plasmid

#86000

Purpose

mammalian expression of HA tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

HA

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSI myc Nup53xl

Plasmid

#86001

Purpose

mammalian expression of myc tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

myc

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-574-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85875

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-574-3p Sensor (MIR574 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-345-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85927

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-345-5p Sensor (MIR345 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-18b-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85896

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-18b-3p Sensor (MIR18B Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-942-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85888

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-942-3p Sensor (MIR942 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-424-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85904

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-424-3p Sensor (MIR424 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-766-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85918

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-766-3p Sensor (MIR766 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-379-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85852

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-379-3p Sensor (MIR379 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.GFP.miR-589-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85911

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-589-5p Sensor (MIR589 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-138-2-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85897

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Inserts

miR-138-3-3p Sesnor
miR-138-2-3p Sensor (MIR138-2 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-615-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85895

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-615-3p Sensor (MIR615 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-769-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85901

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-769-5p Sensor (MIR769 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-340-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85862

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-340-3p Sensor (MIR340 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-455-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85909

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-455-3p Sensor (MIR455 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-323b-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85928

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-323b-5p Sensor (MIR323B Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-324-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85902

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-324-5p Sensor (MIR324 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-877-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85860

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-877-5p (MIR877 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-345-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85886

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-345-3p Sensor (MIR345 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-523-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85903

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-523-3p Sensor (MIR523 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-491-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85925

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-491-5p Sensor (MIR491 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-370-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85916

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-370-3p Sensor (MIR370 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-19a-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85869

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-19a-5p Sensor (MIR19A Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-874-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85859

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-874-5p Sensor (MIR874 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-615-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85913

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-615-3p (MIR615 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-33a-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85853

Purpose

Sensor Plsmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-33a-3p Sensor (MIR33A Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCh-1478

Plasmid

#82424

Purpose

Plasmid for expression of GST-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Tags

GST

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA27_no stop

Plasmid

#83860

Purpose

Gateway donor vector for IAA27 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA27 (PAP2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA8_no stop

Plasmid

#83874

Purpose

Gateway donor vector for IAA8 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 8 (IAA8 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA5_no stop

Plasmid

#83868

Purpose

Gateway donor vector for IAA5 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA5 (IAA5 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA3_no stop

Plasmid

#83863

Purpose

Gateway donor vector for IAA3 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA3 (SHY2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA10_no stop

Plasmid

#83848

Purpose

Gateway donor vector for IAA10 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA4_no stop

Plasmid

#83866

Purpose

Gateway donor vector for IAA4 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA4 (ATAUX2-11 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA28_no stop

Plasmid

#83861

Purpose

Gateway donor vector for IAA28 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

NP_568478.1 (IAA28 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA15_no stop

Plasmid

#83853

Purpose

Gateway donor vector for IAA15 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 15 (IAA15 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry_C1 I3

Plasmid

#61398

Purpose

Expresses mCherry-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3

Plasmid

#61401

Purpose

Expresses GFP-tagged Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
NET33 pEGFP-N2 (594)

Plasmid

#61987

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET33 (SCARA5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCVD419

Plasmid

#63754

Purpose

3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.

Depositor

James Kaper

Article

Levine et al J Infect Dis. 1987 Jul;156(1):175-82.

Insert

ehxA

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 5851 - 5900 of 13335 results

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