We narrowed to 8,825 results for: App

1. pPP441

   Plasmid

   #158801

   Purpose

   Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Tags

   2xSV40NLS-2xFLAG

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG LOC550643 pFCPGW

   Plasmid

   #86853

   Purpose

   retroviral expression of NoBody

   Depositor

   Sarah Slavoff

   Article

   D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

   Insert

   LOC550643 (NBDY )
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5E-hPGK

   Plasmid

   #82579

   Purpose

   5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   Human PGK promoter
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHis-MBP-huTsCas12a

   Plasmid

   #174687

   Purpose

   Expresses 6xHis-MBP tagged TsCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huTsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM6-mCherry

   Plasmid

   #66534

   Purpose

   Expresses mCherry under the control of the Concensus T7 Promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Consensus T7

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPP085

   Plasmid

   #158795

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-elavl3

   Plasmid

   #82400

   Purpose

   Gateway-compatible zebrafish elavl3 promoter

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   elavl3 promoter
   

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xHis-MBP-huMb3Cas12a

   Plasmid

   #174677

   Purpose

   Expresses 6xHis-MBP tagged Mb3Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb3Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-BrainbowTEC

   Plasmid

   #82405

   Purpose

   Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Inserts

   tdTomatoMYC fusion
   EGFP
   E2CrimsonHA
   

   Tags

   HA and MYC

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC_C_AOX

    Plasmid

    #111661

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    AOX
    

    Expression

    Mammalian

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST-KT

    Plasmid

    #44561

    Purpose

    for ectopic inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Promoter

    UV15 promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSFS2A-CaKan

    Plasmid

    #189565

    Purpose

    Codon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selection

    Depositor

    Richard Bennett

    Article

    Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

    Insert

    CaKan
    

    Expression

    Yeast

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cyto-APEX2

    Plasmid

    #215552

    Purpose

    Stable, inducible expression in cytoplasm

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM22082_sfGFP1-10

    Plasmid

    #153515

    Purpose

    E.coli expression plasmid for the production of GFP1-10

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    sfGFP1-10
    

    Expression

    Bacterial

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-syn-ChR2-EYFP-Kv

    Plasmid

    #89256

    Purpose

    Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Insert

    Channelrhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUV15-pHGFP

    Plasmid

    #70045

    Purpose

    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells

    Depositor

    Sabine Ehrt

    Article

    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.

    Insert

    phGFP
    

    Use

    Mycobacterium tuberculosis expression vector

    Promoter

    mycobacterial promoter Psmyc

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6xHis-MBP-huBsCas12a

    Plasmid

    #174671

    Purpose

    Expresses 6xHis-MBP tagged BsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMiniT:SEP-GluA1 TKIT donor

    Plasmid

    #169418

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA1 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRK669

    Plasmid

    #71313

    Purpose

    Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Expression

    Bacterial

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. LeGO-S2

    Plasmid

    #85211

    Purpose

    Lentiviral vector expressing T-Sapphire fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    T-Sapphire
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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