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pGL2B -158/+64

Plasmid

#24946

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pHES874

Plasmid

#89196

Purpose

Expresses mCherry under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-mCherry

Expression

Yeast

Promoter

pZ

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-KpAvs4

Plasmid

#188807

Purpose

Codon optimized KpAvs4 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCDF-EcAvs2

Plasmid

#188804

Purpose

Codon optimized EcAvs2 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-SeAvs1

Plasmid

#188803

Purpose

Codon optimized SeAvs1 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs1

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 1

Plasmid

#8470

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

194YDC196-AAA (Y194A, D195A, C196A)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pFETCh_ONECUT2

Plasmid

#135738

Purpose

Donor vector for 3' FLAG tag of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 Homology arms (ONECUT2 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-QF2

Plasmid

#122563

Purpose

Integration of QF2 sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-QF2-SV40pA

Use

Cloning

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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BASIC_7_J23101-RBS34-mCherry-B0015

Plasmid

#68141

Purpose

storage vector for BASIC part: RFP expression cassette

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23101-RBS34-mcherry-B0015

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 9

Plasmid

#8476

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only
pHES834

Plasmid

#87947

Purpose

Expresses mKate2 under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-mKate2

Use

Synthetic Biology

Expression

Yeast

Promoter

pGal1

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-AtAEP2

Plasmid

#159529

Purpose

Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Arabidopsis thaliana asparaginyl endopeptidase 2

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-CeAEP1

Plasmid

#159528

Purpose

Expresses jack bean (Canavalia ensiformis) asparaginyl endopeptidase 1 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Canavalia ensiformis asparaginyl endopeptidase 1

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGM-ENO2-mCherry-YFP

Plasmid

#180570

Purpose

Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

Depositor

Joel McManus

Article

May et al Elife. 2023 May 25;12:e69611. doi: 10.75

Type

Empty backbone

Expression

Yeast

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 4

Plasmid

#8474

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

391SQ392-PA (S391P, Q392A)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pGM-ENO2-YFP-mCherry

Plasmid

#180569

Purpose

Dual fluorescent reporter for 5' UTR activity in yeast YFP/mCherry

Depositor

Joel McManus

Article

May et al Elife. 2023 May 25;12:e69611. doi: 10.75

Type

Empty backbone

Expression

Yeast

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pUC18-iSpinach

Plasmid

#186053

Purpose

template for co-transcriptional RNA folding assays

Depositor

Markus Wahl

Article

Huang et al Mol Cell. 2020 Sep 17;79(6):1024-1036.e5

Insert

T7A1-rrnG-iSpinach

Tags

no

Expression

Bacterial

Promoter

T7A1

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huLb5Cas12a

Plasmid

#174684

Purpose

Expresses 6xHis-MBP tagged Lb5Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huLb5Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huHkCas12a

Plasmid

#174683

Purpose

Expresses 6xHis-MBP tagged HkCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huHkCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2B -376/+64

Plasmid

#24945

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only