We narrowed to 7,518 results for: Ski;

1. pKPY514

   Plasmid

   #62598

   Purpose

   Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

   Depositor

   David Tirrell

   Article

   Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

   Insert

   Thr251Gly-EcPheRS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Thr251Gly

   Promoter

   T5

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB1-A-pBAD-dCas9

   Plasmid

   #190132

   Purpose

   Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMito.iGABASnFR.F102G

   Plasmid

   #112176

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F102G

   Promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-NTOM20-mVenus-Zdk3

   Plasmid

   #81012

   Purpose

   Expression of Zdk1 anchored to mitochondria

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk3
   

   Use

   Tags

   6x His tag, FLAG, NTOM20, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-Muc2D1D2D3cysD1

   Plasmid

   #155214

   Purpose

   Expresses human MUC2 residues 21-1397

   Depositor

   Deborah Fass

   Article

   Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

   Insert

   Muc2 D1D2D3cysD1 (MUC2 Human)
   

   Use

   Tags

   6xHistadine

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Myo 1b-myc

   Plasmid

   #135064

   Purpose

   Expression of rat Myo 1b (Myr 1) in mammalian cells. C-terminal myc-tag.

   Depositor

   Martin Bähler

   Article

   Ruppert et al J Cell Biol. 1993 Mar;120(6):1393-403. d

   Insert

   Myo 1b with 5' UTR (Myo1b Rat)
   

   Use

   Tags

   Linker and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMB1-A-ptet-dCas9

   Plasmid

   #190129

   Purpose

   Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-luxGold

   Plasmid

   #229948

   Purpose

   Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxGold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. SEPT5 WT

   Plasmid

   #27272

   Depositor

   Harish Pant

   Article

   Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

   Insert

   Septin 5 (SEPTIN5 Human)
   

   Use

   Tags

   Myc (MASMQKLISEEDL)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pALPS puro miR30-L1221

    Plasmid

    #101335

    Purpose

    negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA_e-2

    Plasmid

    #202097

    Purpose

    Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. ER-GCaMP6f-AAV

    Plasmid

    #182501

    Purpose

    GFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotor

    Depositor

    Chris Richards

    Article

    Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

    Insert

    ER localized P450
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Ace2_D92N_E199V-mRuby3

    Plasmid

    #129257

    Purpose

    Express Ace2_D92N_E199V-mRuby3 in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available Since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDCC5

    Plasmid

    #59984

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    D10A

    Promoter

    hsp70Bb

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2-Tbait-UAS-Positron-ST

    Plasmid

    #129266

    Purpose

    UAS-driven expression of Positron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Positron-ST
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    D92N, E199V

    Promoter

    Available Since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEIAV-SIN6.1 CBLacZ-LucWm1A

    Plasmid

    #44167

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Inserts

    LacZ
    firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    CB, CMV enhancer/chicken beta-actin promoter

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2 xDvl-RFP

    Plasmid

    #29683

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    dishevelled (dvl2.L Frog)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-GFPmG1

    Plasmid

    #50444

    Purpose

    Expression of a EGFP variant in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GFPmG1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E143K, N147Q

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET42a-lambdaN+-L+-GSH

    Plasmid

    #98894

    Purpose

    Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

    Depositor

    Pascale Legault

    Article

    Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

    Insert

    lambdaN+-L+
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAC-C-GFP

    Plasmid

    #172973

    Purpose

    MAC-tagged GFP expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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