We narrowed to 13,760 results for: TIM

1. pSox2-bd::FP

   Plasmid

   #34703

   Depositor

   Hollis Cline

   Article

   Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

   Insert

   eGFP
   

   Use

   Xenopus expression

   Tags

   165-bp minimal FGF4 promoter, 6x Sox2/Oct3-4 tran…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. OeNL-H2B_pcDNA3

   Plasmid

   #89527

   Purpose

   Orange color variant of bright luminescent protein, fused to histone 2B

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Use

   Tags

   histone 2B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-U6-crRNA1

   Plasmid

   #123639

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   crRNA scaffold 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAW8ENdeI-cHAIAA7-spo5DSR

   Plasmid

   #49873

   Purpose

   for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   IAA17 degron tag

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. RV-Syn-Dio-GFP

   Plasmid

   #87673

   Purpose

   retroviral vector encoding cre dependent expression of GFP

   Depositor

   Oscar Marin

   Article

   Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. KA1130_pPB-TREtight-cHA-IRESH2BmCherry

   Plasmid

   #124173

   Purpose

   piggyBac-based Dox-inducible expression

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiCRISPRv1.linker_KO

   Plasmid

   #164688

   Purpose

   For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   miR-144~451 linker CRISPR KO gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUPER-retro-puro-shNup133-2

   Plasmid

   #87333

   Purpose

   To express shRNA against human Nup133

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup133
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. OeNL-Actin_pcDNA3

   Plasmid

   #89524

   Purpose

   Orange color variant of bright luminescent protein, fused to actin

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Use

   Tags

   Actin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMOS030: ratiometric pHluorin (endoplasmic reticulum)

    Plasmid

    #163054

    Purpose

    ratiometric pHluorin (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    ratiometric pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET FLII32Pglu-2.2m

    Plasmid

    #13566

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A P32S-ECFP-D33R
    

    Use

    Tags

    6xHis and Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ414 - DANCER-3

    Plasmid

    #136387

    Purpose

    Expresses DANCER-3 dynamic protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    DANCER-3
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39L-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCBB-lacZa

    Plasmid

    #32563

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    LacZ-alpha
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. KA2706_eSpCas9-PGKPacdtkCh

    Plasmid

    #124204

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYFP-x-1

    Plasmid

    #140142

    Purpose

    Evaluating information transmission of a genetic sensor.

    Depositor

    Drew Tack

    Article

    Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

    Insert

    lacY (lacY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D37, I38, and N39 are changed to nonsense (stop) …

    Promoter

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ158

    Plasmid

    #69307

    Purpose

    Gateway entry vector with hSpCas9 (Human codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9 (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSox2-bd_mFGF_eGFP

    Plasmid

    #34704

    Depositor

    Hollis Cline

    Article

    Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

    Insert

    eGFP
    

    Use

    Xenopus expression

    Tags

    165-bp minimal FGF4 promoter and 6x Sox2/Oct3-4 t…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEmerald-PCNA-19-NLS-4

    Plasmid

    #54222

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Use

    Tags

    SV40NLS and mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. B3rec.zf1

    Plasmid

    #108354

    Purpose

    Zebrafish codon optimized B3 recombinase that acts on blown-out sites

    Depositor

    Harold Burgess

    Article

    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

    Insert

    B3 recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Zebrafish codon optimized

    Promoter

    lac

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNB763

    Plasmid

    #60158

    Purpose

    Firefly luciferase (Promega) driven by LYS9 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LYS9pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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