163,740 results

1. pCMV-sadCas9-VP64

   Plasmid

   #115790

   Purpose

   An AAV vector expressing S. aureus dCas9 fused to VP64.

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   Sa-dCas9
   

   Use

   AAV

   Tags

   NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-ERT2CreERT2

   Plasmid

   #13777

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   ERT2-Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CMV-jGCaMP8s

   Plasmid

   #162371

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8s
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-mitoGFP

   Plasmid

   #44385

   Depositor

   Pantelis Tsoulfas

   Article

   Kitay et al Hum Mol Genet. 2013 Jan 24.

   Insert

   mitoGFP (COX8A Human)
   

   Use

   Lentiviral

   Tags

   Cox8 targeting sequence and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-iCas9-neo

   Plasmid

   #85400

   Purpose

   Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Insert

   Flag-iCas9-P2A-GFP
   

   Use

   Lentiviral

   Tags

   Flag-iCas9-P2A-GFP

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-N-His

   Plasmid

   #119755

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Use

   Tags

   10xHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICH47742:: pRPS5a-PE2max-NC::tHSP

   Plasmid

   #227694

   Purpose

   Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   PE2max-NC being driven by pRPS5a and terminated by tHSP
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pRPS5a

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTP955

   Plasmid

   #104164

   Purpose

   Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBES1-LacOx50-mCh-bla

    Plasmid

    #210260

    Purpose

    Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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