We narrowed to 9,416 results for: Coli

1. pBEL2077

   Plasmid

   #155157

   Purpose

   Expresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   MlaF_E170Q

   Promoter

   T7 promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEL2078

   Plasmid

   #155158

   Purpose

   Expresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   MlaF_Y261A

   Promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBEL2079

   Plasmid

   #155159

   Purpose

   Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   MlaF_L265A

   Promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEL2042

   Plasmid

   #139927

   Purpose

   Complementation plasmid for LetAB with 4 ring (rings 1347) LetB mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB (1-159; 280-515; 747-877)

   Promoter

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL2045

   Plasmid

   #139924

   Purpose

   Expression plasmid for 4 ring (rings 1347) LetB (without TM region) mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB (43-159; 280-515; 747-877)

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL2002

   Plasmid

   #139887

   Purpose

   Expression plasmid for LetAB with 4 ring (rings 1567) LetB (without TM region) mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta161-514)-6xHis

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEL2001

   Plasmid

   #139886

   Purpose

   Expression plasmid for LetAB with 3 ring (rings 167) LetB (without TM region) mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta161-634)-6xHis

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29b-clbI S178A

   Plasmid

   #114159

   Purpose

   Expresses ClbI S178A

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbI
   

   Use

   Tags

   6xHis and 6xHis-Thrombin site

   Expression

   Bacterial

   Mutation

   Mutated S178 to Ala

   Promoter

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH861-gst-optec P210T

   Plasmid

   #89595

   Purpose

   Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed proline 210 to threonine

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTH846-gst-dissc

    Plasmid

    #96907

    Purpose

    Plasmid for yeast expression of GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    yeast codon dis-optimised glutathione-S transferase
    

    Use

    Tags

    8xhistidine

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH860-gst-optec A180Q

    Plasmid

    #89594

    Purpose

    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed alanine 180 to glutamine

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTH858-gst-optec V156E

    Plasmid

    #89592

    Purpose

    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed valine 156 to glutamic acid

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTH859-gst-optec Y172M

    Plasmid

    #89593

    Purpose

    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed tyrosine 172 to methionine

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKSJ375

    Plasmid

    #103064

    Purpose

    Expresses ImmE1, immunity to colicin E1

    Depositor

    Alan Finkelstein, Karen Jakes

    Article

    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

    Insert

    colicin E1 immunity protein (pColE1_26, imm E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E1 immunity protein promoter from ColE1

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ppj219(JBEI-13475)

    Plasmid

    #89785

    Purpose

    for expression of N-StrepII-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ppj184 (JBEI-13431)

    Plasmid

    #89784

    Purpose

    for expression of N-His-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ppj187 (JBEI-13437)

    Plasmid

    #89783

    Purpose

    for expression of C-His-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPJ169(JPUB_006769)

    Plasmid

    #83835

    Purpose

    Carries NEW operon 5 final

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Inserts

    Kuste3340
    Kuste3350
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    op5_3350_for and op5_BsaI_3340_for

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPJ170(JPUB_006771)

    Plasmid

    #83833

    Purpose

    Carries NEW operon 6 final

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Inserts

    Kuste3346
    Kuste3348
    Kuste3349
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    op6_3348_for, op6_3349_for, and op6_BsmBI_3346_fo…

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPJ168(JPUB_006767)

    Plasmid

    #83832

    Purpose

    Carries NEW operon 4 final

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Inserts

    Kuste2802
    Kuste2805
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    op4_2805_for and op4_BsaI_2802_for

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBP-BBa_B0012

    Plasmid

    #72997

    Purpose

    BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Bba_B0012 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBP-BBa_B0015

    Plasmid

    #72998

    Purpose

    BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    BBa_B0015 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTU2_2b

    Plasmid

    #73011

    Purpose

    Secondary module (BpiI) site in pTU2b backbone

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Secondary module + Golden Gate pTU2b BsmBI destination site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBP-ORF-eCFP

    Plasmid

    #72961

    Purpose

    Level 0 Entry vector" ORF - eCFP"

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Enhanced cyan fluorescent protein (eCFP)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBP-ORF-mCherry

    Plasmid

    #72962

    Purpose

    Level 0 Entry vector" ORF - mCherry "

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    mCherry fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBP-SJM902

    Plasmid

    #72967

    Purpose

    Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM902 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pBP-SJM903

    Plasmid

    #72968

    Purpose

    Level 0 - SJM903 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM903 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pBP-SJM905

    Plasmid

    #72969

    Purpose

    Level 0 - SJM905 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM905 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pBP-SJM906

    Plasmid

    #72970

    Purpose

    Level 0 - SJM906 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM906 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pBP-SJM908

    Plasmid

    #72971

    Purpose

    Level 0 - SJM908 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM908 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only